In vitro 3D co-culture model of human endothelial and smooth muscle cells to study pathological vascular remodeling.

Autor:	San Sebastián-Jaraba I; Laboratorio de Patología Vascular, IIS-Fundación Jiménez Díaz, Madrid, España; CIBER de Enfermedades Cardiovasculares (CIBERCV), España., Fernández-Gómez MJ; Laboratorio de Patología Vascular, IIS-Fundación Jiménez Díaz, Madrid, España., Blázquez-Serra R; Laboratorio de Patología Vascular, IIS-Fundación Jiménez Díaz, Madrid, España; CIBER de Enfermedades Cardiovasculares (CIBERCV), España., Sanz-Andrea S; Laboratorio de Patología Vascular, IIS-Fundación Jiménez Díaz, Madrid, España., Blanco-Colio LM; Laboratorio de Patología Vascular, IIS-Fundación Jiménez Díaz, Madrid, España; CIBER de Enfermedades Cardiovasculares (CIBERCV), España., Méndez-Barbero N; Laboratorio de Patología Vascular, IIS-Fundación Jiménez Díaz, Madrid, España; CIBER de Enfermedades Cardiovasculares (CIBERCV), España; Facultad de Medicina y Biomedicina, Universidad Alfonso X el Sabio, Madrid, España. Electronic address: nerea.mendez@quironsalud.es.
Jazyk:	English; Spanish; Castilian
Zdroj:	Clinica e investigacion en arteriosclerosis : publicacion oficial de la Sociedad Espanola de Arteriosclerosis [Clin Investig Arterioscler] 2024 Nov-Dec; Vol. 36 (6), pp. 356-363. Date of Electronic Publication: 2024 May 08.
DOI:	10.1016/j.arteri.2024.03.007
Abstrakt:	Pathological vascular remodeling of the vessel wall refers to the structural and functional changes of the vessel wall that occur in response to injury that eventually leads to cardiovascular disease. The vessel wall is composed of two main types of cells, endothelial cells and vascular smooth muscle cells, whose communication is crucial in both the development of the vasculature and the homeostasis of mature vessels. Changes in the dialogue between endothelial cells and vascular smooth muscle cells are associated with various pathological states that triggers remodeling of the vascular wall. For many years, considerable efforts have been made to develop effective diagnoses and treatments for these pathologies by studying their mechanisms in both in vitro and in vivo models. Compared to animal models, in vitro models can provide great opportunities to obtain data in a more homogeneous, economical and massive way, providing an overview of the signaling pathways responsible for these pathologies. The implementation of three-dimensional in vitro co-culture models for the study of other pathologies has been postulated as a potentially applicable methodology, which determines the importance of its application in studies of cardiovascular diseases. In this article we present a method for culturing human endothelial cells and vascular smooth muscle cells, grown under non-adherent conditions, that generate three-dimensional spheroidal structures with greater physiological equivalence to in vivo conditions. This in vitro modeling could be used as a study tool to identify cellular and molecular mechanisms involved in the pathological processes underlying vascular remodeling. (Copyright © 2024 The Author(s). Publicado por Elsevier España, S.L.U. All rights reserved.)
Databáze:	MEDLINE
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