Cytotoxicity effects and differentiation potential of ormocer-based and nanohybrid composite resins on human dental pulp stem cells.

Autor: Del Giudice C; Department of Neuroscience, Reproductive Sciences and Dentistry, University of Naples Federico II, Naples, Italy., Rengo C; Department of Neuroscience, Reproductive Sciences and Dentistry, University of Naples Federico II, Naples, Italy., Maglitto M; Department of Neuroscience, Reproductive Sciences and Dentistry, University of Naples Federico II, Naples, Italy., Armogida NG; Department of Neuroscience, Reproductive Sciences and Dentistry, University of Naples Federico II, Naples, Italy., Iaculli F; Department of Neuroscience, Reproductive Sciences and Dentistry, University of Naples Federico II, Naples, Italy. Electronic address: flavia.iaculli@unina.it., Rengo S; Department of Neuroscience, Reproductive Sciences and Dentistry, University of Naples Federico II, Naples, Italy., Menale C; Department of Clinical Medicine and Surgery, University of Naples 'Federico II', Naples, Italy., Spagnuolo G; Department of Neuroscience, Reproductive Sciences and Dentistry, University of Naples Federico II, Naples, Italy. Electronic address: gspagnuo@unina.it.
Jazyk: angličtina
Zdroj: Dental materials : official publication of the Academy of Dental Materials [Dent Mater] 2024 Jun; Vol. 40 (6), pp. 984-992. Date of Electronic Publication: 2024 May 09.
DOI: 10.1016/j.dental.2024.05.011
Abstrakt: Objective: to compare conventional nanohybrid (Ceram.x Spectra) and ormocer-based (Admira fusion) dental composite resins effects on human dental pulp stem cells (hDPSCs) in terms of cytotoxicity, self-renewal, migration and osteogenic differentiation.
Methods: hDPSCs were cultured in presence of different dilutions (undiluted, form 1:2 to 1:100) of CeramX (CX) and Admira fusion (AD) eluates and viability assay in standard or osteogenic conditions were performed. Samples and eluates were prepared according to ISO 10993-12. In addition, apoptosis, self-renewal and migration activity evaluations were carried out. Osteogenic differentiation potential was tested by Alkaline Phosphatase Activity, alizarin red staining and gene expression of specific markers (ALP, RUNX2, OCN, OPN and COL1α1). Statistical analysis was performed by means of a One-way analysis of variance (One-way ANOVA) followed by a Tukey's test for multiple comparison; results were presented as mean ± standard error of mean (SEM).
Results: Admira Fusion demonstrated to be highly biocompatible and showed positive effects on hDPSCs proliferation and differentiation; on the contrary, conventional nanohybrid composite showed to be more cytotoxic and without any notable effect on stem cells differentiation. Moreover, the obtained results were further corroborated by a significant upregulation of osteogenic differentiation markers obtained in presence of ormocer-based composite resin eluate. Specifically, in AD 1:50 group expression levels of ALP, Runx2, Col1α1 were double than control (ALP, p = 0.045; Runx2, p = 0.003; Col1α1, p = 0.001) and CX 1:50 (ALP, p = 0.006; RUNX2, p = 0.029; Col1α1, p = 0.005). Moreover, in the same group, OPN and OCN resulted about 5 times more expressed as compared to control (OPN, p = 0.009; OCN, p = 0.0005) and CX 1:50 (OPN, p = 0.012; OCN, p = 0.0006).
Significance: The less cytotoxicity obtained by AD than conventional nanohybrid composite may be attributed to a reduced monomers release in the oral environment, supporting the hypothesis of limited adverse effect and enhanced healing potential, mainly when the material is positioned in close contact with pulp tissue.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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