ToF-SIMS imaging shows specific lipophilic vitamin alterations in chronic reprotoxicity caused by the emerging contaminant Pravastatin in Gammarus fossarum.

Autor: Fu T; Univ Lyon, CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, Villeurbanne F-69100, France., Calabrese V; Univ Lyon, CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, Villeurbanne F-69100, France., Bancel S; INRAEe, UR RiverLy, Ecotoxicology Team, Villeurbanne F-69625, France., Quéau H; INRAEe, UR RiverLy, Ecotoxicology Team, Villeurbanne F-69625, France., Garnero L; INRAEe, UR RiverLy, Ecotoxicology Team, Villeurbanne F-69625, France., Delorme N; INRAEe, UR RiverLy, Ecotoxicology Team, Villeurbanne F-69625, France., Abbaci K; INRAEe, UR RiverLy, Ecotoxicology Team, Villeurbanne F-69625, France., Salvador A; Univ Lyon, CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, Villeurbanne F-69100, France., Chaumot A; INRAEe, UR RiverLy, Ecotoxicology Team, Villeurbanne F-69625, France., Geffard O; INRAEe, UR RiverLy, Ecotoxicology Team, Villeurbanne F-69625, France., Degli-Esposti D; INRAEe, UR RiverLy, Ecotoxicology Team, Villeurbanne F-69625, France. Electronic address: davide.degli-esposti@inrae.fr., Ayciriex S; Univ Lyon, CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, Villeurbanne F-69100, France. Electronic address: sophie.ayciriex@univ-lyon1.fr.
Jazyk: angličtina
Zdroj: Aquatic toxicology (Amsterdam, Netherlands) [Aquat Toxicol] 2024 Jun; Vol. 271, pp. 106935. Date of Electronic Publication: 2024 Apr 29.
DOI: 10.1016/j.aquatox.2024.106935
Abstrakt: Blood lipid-lowering agents, such as Pravastatin, are among the most frequently used pharmaceuticals released into the aquatic environment. Although their effects on humans are very well understood, their consequences on freshwater organisms are not well known, especially in chronic exposure conditions. Gammarus fossarum is commonly used as sentinel species in ecotoxicology because of its sensitivity to a wide range of environmental contaminants and the availability of standardized bioassays. Moreover, there is an increased interest in linking molecular changes in sentinel species, such as gammarids, to observed toxic effects. Here, we performed a reproductive toxicity assay on females exposed to different concentrations of pravastatin (30; 300; 3,000 and 30,000 ng L -1 ) during two successive reproductive cycles and we applied ToF-SIMS imaging to evaluate the effect of pravastatin on lipid homeostasis in gammarids. Reproductive bioassay showed that pravastatin could affect oocyte development in Gammarus fossarum inducing embryotoxicity in the second reproductive cycle. Mass spectrometry imaging highlighted the disruption in vitamin E production in the oocytes of exposed female gammarids at the second reproductive cycle, while limited alterations were observed in other lipid classes, regarding both production and tissue distribution. The results demonstrated the interest of applying spatially resolved lipidomics by mass spectrometry imaging to assess the molecular effects induced by long-term exposure to environmental pharmaceutical residues in sentinel species.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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