Comprehensive analysis of early T cell responses to acute Zika Virus infection during the first epidemic in Bahia, Brazil.

Autor: Samri A; Sorbonne-Université, Inserm 1135, CNRS ERL8255, Centre d'immunologie et des Maladies Infectieuses, Cimi, Paris, France., Bandeira AC; Secretaria de Saúde da Bahia, Salvador, Bahia, Brazil.; Instituto Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Salvador, Brazil., Gois LL; Instituto Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Salvador, Brazil.; Escola Bahiana de Medicina e Saúde Pública (EBMSP), Salvador, Brazil.; Departamento de Biointeração, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Brazil., Silva CGR; Escola Bahiana de Medicina e Saúde Pública (EBMSP), Salvador, Brazil., Rousseau A; Sorbonne-Université, Inserm 1135, CNRS ERL8255, Centre d'immunologie et des Maladies Infectieuses, Cimi, Paris, France., Corneau A; Faculté de Médecine Pierre et Marie Curie, Plateforme de Cytométrie (CyPS), UMS30-LUMIC, Paris, France., Tarantino N; Sorbonne-Université, Inserm 1135, CNRS ERL8255, Centre d'immunologie et des Maladies Infectieuses, Cimi, Paris, France., Maucourant C; Sorbonne-Université, Inserm 1135, CNRS ERL8255, Centre d'immunologie et des Maladies Infectieuses, Cimi, Paris, France., Queiroz GAN; Instituto Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Salvador, Brazil.; Escola Bahiana de Medicina e Saúde Pública (EBMSP), Salvador, Brazil., Vieillard V; Sorbonne-Université, Inserm 1135, CNRS ERL8255, Centre d'immunologie et des Maladies Infectieuses, Cimi, Paris, France., Yssel H; Sorbonne-Université, Inserm 1135, CNRS ERL8255, Centre d'immunologie et des Maladies Infectieuses, Cimi, Paris, France., Campos GS; Departamento de Biointeração, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Brazil., Sardi S; Departamento de Biointeração, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Brazil., Autran B; Sorbonne-Université, Inserm 1135, CNRS ERL8255, Centre d'immunologie et des Maladies Infectieuses, Cimi, Paris, France., Rios Grassi MF; Instituto Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Salvador, Brazil.; Escola Bahiana de Medicina e Saúde Pública (EBMSP), Salvador, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 May 09; Vol. 19 (5), pp. e0302684. Date of Electronic Publication: 2024 May 09 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0302684
Abstrakt: Background: In most cases, Zika virus (ZIKV) causes a self-limited acute illness in adults, characterized by mild clinical symptoms that resolve within a few days. Immune responses, both innate and adaptive, play a central role in controlling and eliminating virus-infected cells during the early stages of infection.
Aim: To test the hypothesis that circulating T cells exhibit phenotypic and functional activation characteristics during the viremic phase of ZIKV infection.
Methods: A comprehensive analysis using mass cytometry was performed on peripheral blood mononuclear cells obtained from patients with acute ZIKV infection (as confirmed by RT-PCR) and compared with that from healthy donors (HD). The frequency of IFN-γ-producing T cells in response to peptide pools covering immunogenic regions of structural and nonstructural ZIKV proteins was quantified using an ELISpot assay.
Results: Circulating CD4+ and CD8+ T lymphocytes from ZIKV-infected patients expressed higher levels of IFN-γ and pSTAT-5, as well as cell surface markers associated with proliferation (Ki-67), activation ((HLA-DR, CD38) or exhaustion (PD1 and CTLA-4), compared to those from HD. Activation of CD4+ and CD8+ memory T cell subsets, including Transitional Memory T Cells (TTM), Effector Memory T cells (TEM), and Effector Memory T cells Re-expressing CD45RA (TEMRA), was prominent among CD4+ T cell subset of ZIKV-infected patients and was associated with increased levels of IFN-γ, pSTAT-5, Ki-67, CTLA-4, and PD1, as compared to HD. Additionally, approximately 30% of ZIKV-infected patients exhibited a T cell response primarily directed against the ZIKV NS5 protein.
Conclusion: Circulating T lymphocytes spontaneously produce IFN-γ and express elevated levels of pSTAT-5 during the early phase of ZIKV infection whereas recognition of ZIKV antigen results in the generation of virus-specific IFN-γ-producing T cells.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Samri et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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