Wired for Success: Probing the Effect of Tissue-Engineered Neural Interface Substrates on Cell Viability.

Autor: Nascimento ATD; ARC Centre of Excellence for Electromaterials Science, School of Science, Computing and Engineering Technologies, Swinburne University of Technology, Melbourne, Victoria 3122, Australia.; The Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Melbourne, Victoria 3065, Australia., Mendes AX; ARC Centre of Excellence for Electromaterials Science, School of Science, Computing and Engineering Technologies, Swinburne University of Technology, Melbourne, Victoria 3122, Australia.; The Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Melbourne, Victoria 3065, Australia., Duchi S; The Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Melbourne, Victoria 3065, Australia.; Department of Surgery, University of Melbourne, St Vincent's Hospital, Melbourne, Victoria 3065, Australia., Duc D; School of Pharmacy and Pharmaceutical Sciences, College of Biomedical and Life Sciences, Cardiff University, Cardiff CF10 3NB, United Kingdom., Aguilar LC; The Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Melbourne, Victoria 3065, Australia.; The Graeme Clark Institute, The University of Melbourne, Melbourne, Victoria 3010, Australia.; Department of Biomedical Engineering, Faculty of Engineering and Information Technology, The University of Melbourne, Melbourne, Victoria 3010, Australia., Quigley AF; The Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Melbourne, Victoria 3065, Australia.; School of Electrical and Biomedical Engineering, RMIT University, Melbourne, Victoria 3001, Australia.; Department of Medicine, University of Melbourne, St Vincent's Hospital Melbourne, Melbourne, Victoria 3065, Australia., Kapsa RMI; The Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Melbourne, Victoria 3065, Australia.; School of Electrical and Biomedical Engineering, RMIT University, Melbourne, Victoria 3001, Australia.; Department of Medicine, University of Melbourne, St Vincent's Hospital Melbourne, Melbourne, Victoria 3065, Australia., Nisbet DR; The Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Melbourne, Victoria 3065, Australia.; The Graeme Clark Institute, The University of Melbourne, Melbourne, Victoria 3010, Australia.; Department of Biomedical Engineering, Faculty of Engineering and Information Technology, The University of Melbourne, Melbourne, Victoria 3010, Australia.; Melbourne Medical School, Faculty of Medicine, Dentistry and Health Science, The University of Melbourne, Melbourne, Victoria 3010, Australia., Stoddart PR; School of Science, Computing and Engineering Technologies, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia., Silva SM; Department of Chemistry and Biochemistry, La Trobe Institute for Molecular Science, The Biomedical and Environmental Sensor Technology Research Centre, La Trobe University, Melbourne, Victoria 3086, Australia., Moulton SE; ARC Centre of Excellence for Electromaterials Science, School of Science, Computing and Engineering Technologies, Swinburne University of Technology, Melbourne, Victoria 3122, Australia.; The Aikenhead Centre for Medical Discovery, St Vincent's Hospital Melbourne, Melbourne, Victoria 3065, Australia.; Iverson Health Innovation Research Institute, Swinburne University of Technology, Melbourne, Victoria 3122, Australia.
Jazyk: angličtina
Zdroj: ACS biomaterials science & engineering [ACS Biomater Sci Eng] 2024 Jun 10; Vol. 10 (6), pp. 3775-3791. Date of Electronic Publication: 2024 May 09.
DOI: 10.1021/acsbiomaterials.4c00111
Abstrakt: This study investigates the electrochemical behavior of GelMA-based hydrogels and their interactions with PC12 neural cells under electrical stimulation in the presence of conducting substrates. Focusing on indium tin oxide (ITO), platinum, and gold mylar substrates supporting conductive scaffolds composed of hydrogel, graphene oxide, and gold nanorods, we explored how the substrate materials affect scaffold conductivity and cell viability. We examined the impact of an optimized electrical stimulation protocol on the PC12 cell viability. According to our findings, substrate selection significantly influences conductive hydrogel behavior, affecting cell viability and proliferation as a result. In particular, the ITO substrates were found to provide the best support for cell viability with an average of at least three times higher metabolic activity compared to platinum and gold mylar substrates over a 7 day stimulation period. The study offers new insights into substrate selection as a platform for neural cell stimulation and underscores the critical role of substrate materials in optimizing the efficacy of neural interfaces for biomedical applications. In addition to extending existing work, this study provides a robust platform for future explorations aimed at tailoring the full potential of tissue-engineered neural interfaces.
Databáze: MEDLINE