Systematic review and meta-analysis of cryopreserved bovine sperm assessment: harnessing imaging flow cytometry for multi-parametric analysis.

Autor:	Umirbaeva A; Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, Kazakhstan., Kurenkov A; Department of Computer Sciences, School of Engineering and Digital Sciences, Nazarbayev University, Astana, Kazakhstan., Makhanbetova A; JSC 'Republican Center of Breeding in Livestock' 'Asyl-Tulik', Kosshi, Kazakhstan., Seisenov B; JSC 'Republican Center of Breeding in Livestock' 'Asyl-Tulik', Kosshi, Kazakhstan., Vorobjev IA; Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, Kazakhstan., Barteneva NS; Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, Kazakhstan.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2024 Apr 24; Vol. 11, pp. 1371586. Date of Electronic Publication: 2024 Apr 24 (Print Publication: 2024).
DOI:	10.3389/fvets.2024.1371586
Abstrakt:	Cryopreservation of sperm is an essential technique in assisted reproduction in cattle. The objective of the study was to systematically review and synthesize the literature on bull semen quality evaluation based on the comparison of morphological and metabolic parameters of cryopreserved bovine spermatozoa such as DNA integrity, mitochondrial status, plasma membrane alterations, total motility, and morphology (% of abnormal cells). The electronic databases PubMed, Web of Sciences, Scopus, and Google Scholar were searched up to December 2023. Studies and references were included if they reported the following parameters: DNA integrity, mitochondrial status, plasma membrane alterations, total motility, and morphological aberrations (% of abnormal cells) for conventional cryopreserved bovine spermatozoa. After an electronic search, out of 1,526 original studies, only 40 were included in the meta-analysis. Standardized mean differences (SMD) with 95% confidence intervals were estimated for the chosen studies, and a meta-analysis was performed using a random effects model. The tau-squared (tau 2 ) and inconsistency index ( I 2 ) quantified heterogeneity among different studies. The regression analysis for the evaluated parameters showed a positive correlation between mitochondrial membrane potential (MMP), total motility, and abnormal morphology and a negative correlation between DNA fragmentation index (DFI) and total motility and MMP. Moreover, subgroup analysis demonstrated similar associations for dairy and non-dairy bull breeds, albeit with lower I 2 values. The presence of publication bias was confirmed by Egger's test, except for the MMP parameter. A multi-parametric analysis of morphological and metabolic parameters can address the existing limitations of cryopreserved bovine spermatozoa quality assessment. Combining imaging flow cytometry (IFC) with standardization of sperm pre-processing and optimization of the experimental protocols may help to differentiate sperm from cellular debris and cytoplasmic droplets of similar size and alleviate limitations demonstrated by conventional sperm analysis. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Umirbaeva, Kurenkov, Makhanbetova, Seisenov, Vorobjev and Barteneva.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu