Correlation between the results of cultures and the molecular BIOFIRE® joint infection panel in a cohort of pediatric patients with bone and joint infections in Bogotá, Colombia.
| Autor: | Camacho-Moreno G; HOMI, Fundación Hospital Pediátrico la Misericordia, Bogotá, Colombia.; Departamento de Pediatria, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia., Vergara-Amador E; HOMI, Fundación Hospital Pediátrico la Misericordia, Bogotá, Colombia.; Unidad de Ortopedia, Departamento de Cirugía, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia., Martínez-Villegas T; Unidad de Ortopedia, Departamento de Cirugía, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia., Aragón-Joya Y; Departamento de Pediatria, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia., Romero-Cardozo L; Unidad de Ortopedia, Departamento de Cirugía, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia., Lores-Garcia F; Unidad de Ortopedia, Departamento de Cirugía, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia., Moreno VM; HOMI, Fundación Hospital Pediátrico la Misericordia, Bogotá, Colombia., Leal-Castro AL; Departamento de Microbiología, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in pediatrics [Front Pediatr] 2024 Apr 24; Vol. 12, pp. 1359736. Date of Electronic Publication: 2024 Apr 24 (Print Publication: 2024). |
| DOI: | 10.3389/fped.2024.1359736 |
| Abstrakt: | Introduction: Bone and Joint Infections (BJI) have high morbidity. Methicillin resistant Staphylococcus aureus (MRSA) has increased. Culture-based diagnosis has difficult to recovering fastidious bacteria and detecting polymicrobial infections, molecular methods offer a promising improvement for the diagnosis of BJI with reduced time to result. The aim of the study was to determine the correlation between culture results and the Biofire joint infection panel (BJIP) in a cohort of pediatric patients with BJI. Materials and Methods: Descriptive study. Patients admitted with probable o confirmed BJI between July 1, 2019 and February 28, 2021 at HOMI. Blood cultures, synovial and bone fluid samples were taken. Samples were kept at -70 °C. On September 2022, the panel was performed. Results: 32 patients were included . The average age was 83m (RIQ: 32-145). 23 (71.8%) patients had a positive culture. The most frequent microorganism were S. aureus 19 (83%), 11/19 (57.9%) Staphylococci isolates were MRSA. 24/32 (75%) were positive by panel, 20 positive detections were concordant with culture, there were 6 additional isolates by panel (2 S. aureus , 2 S. pyogenes , 1 K. kingae and 1 C. albicans ), three microorganisms were isolated in culture but not in the panel. (2 S. aureus and 1 S. agalactiae ). Two patients with coinfection were detected. All MRSA were detected by culture and panel. In 26 (81.3%) patients the etiology was documented by any method. Conclusion: These results showed a moderate level of agreement between BJIP and culture ( κ = 0.47). The panel allowed the detection of fastidious bacteria including K. kingae and polymicrobial samples. There was a very good level of agreement between the panel and culture for the MRSA detection ( κ = 1). Competing Interests: The molecular panels were donated by bioMérieux and they did not participate in the design or analysis of the study results. GC-M: Has received support from Pfizer, MSD (Merck Sharp and Dohme) for participation in congresses and paid conferences, has participated in advisory boards and has received support from for research. He has received fees from BiomerieuxTM for lectures. AL-C: Has received support from Pfizer, MSD (Merck Sharp and Dohme) for participation in congresses and paid conferences, has participated in advisory boards and has received support from for research. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (© 2024 Camacho-Moreno, Vergara-Amador, Martínez-Villegas, Aragón-Joya, Romero-Cardozo, Lores-Garcia, Moreno and Leal-Castro.) |
| Databáze: | MEDLINE |
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