Development and evaluation of time-resolved rapid immunofluorescence test for detection of TSOL18 specific antibody in porcine cysticercosis infections.

Autor: Zhang D; College of Animal Science and Technology, Inner Mongolia MinZu University, Tongliao, 028000, China., Duan R; College of Animal Science and Technology, Inner Mongolia MinZu University, Tongliao, 028000, China., Liu J; Qingdao Special Servicemen Recuperation Center of PLA Navy, Qingdao, 266071, China., Wang M; College of Animal Science and Technology, Inner Mongolia MinZu University, Tongliao, 028000, China., Yang Y; College of Veterinary Medicine, Yunnan Agricultural University, Kunming, 650201, China., Zhao Y; College of Animal Science and Technology, Inner Mongolia MinZu University, Tongliao, 028000, China., Liu M; State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, 130062, China. liumy36@163.com.; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225000, China. liumy36@163.com., Sun S; College of Veterinary Medicine, Yunnan Agricultural University, Kunming, 650201, China. shums1975@163.com.
Jazyk: angličtina
Zdroj: BMC veterinary research [BMC Vet Res] 2024 May 08; Vol. 20 (1), pp. 182. Date of Electronic Publication: 2024 May 08.
DOI: 10.1186/s12917-024-04034-7
Abstrakt: Background: Porcine cysticercosis, a serious zoonotic parasitic disease, is caused by the larvae of Taenia solium and has been acknowledged by the World Organization for Animal Health. The current detection methods of Cysticercus cellulosae cannot meet the needs of large-scale and rapid detection in the field. We hypothesized that the immunofluorescence chromatography test strip (ICS) for detecting Cysticercus cellulosae, according to optimization of a series of reaction systems was conducted, and sensitivity, specificity, and stability testing, and was finally compared with ELISA. This method utilizes Eu 3+ -labeled time-resolved fluorescent microspheres (TRFM) coupled with TSOL18 antigen to detect TSOL18 antibodies in infected pig sera.
Results: ICS and autopsy have highly consistent diagnostic results (n = 133), as determined by Cohen's κ analysis (κ = 0.925). And the results showed that the proposed ICS are high sensitivity (0.9459) with specificity (0.9792). The ICS was unable to detect positive samples of other parasites. It can be stored for at least six months at 4℃.
Conclusions: In summary, we established a TRFM-ICS method with higher sensitivity and specificity than indirect ELISA. Results obtained from serum samples can be read within 10 min, indicating a rapid, user-friendly test suitable for large-scale field detection.
(© 2024. The Author(s).)
Databáze: MEDLINE
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