Construction of dentin-on-a-chip based on microfluidic technology and tissue engineering.

Autor: Zhang H; Center for Microscope Enhanced Dentistry, School of Stomatology, Capital Medical University, Beijing 100162, PR China; Department of Endodontics and Operative Dentistry, School of Stomatology, Capital Medical University, Beijing 100050, PR China., Li L; Wenzhou Institute University of Chinese Academy of Sciences, Wenzhou 325001, PR China. Electronic address: lilingjun@ucas.ac.cn., Wang S; Wenzhou Institute University of Chinese Academy of Sciences, Wenzhou 325001, PR China., Sun X; Department of Endodontics and Operative Dentistry, School of Stomatology, Capital Medical University, Beijing 100050, PR China., Luo C; Wenzhou Institute University of Chinese Academy of Sciences, Wenzhou 325001, PR China; The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics, Peking University, Beijing 100871, PR China. Electronic address: pkuluocx@pku.edu.cn., Hou B; Center for Microscope Enhanced Dentistry, School of Stomatology, Capital Medical University, Beijing 100162, PR China. Electronic address: houbenxiang@gmail.com.
Jazyk: angličtina
Zdroj: Journal of dentistry [J Dent] 2024 Jul; Vol. 146, pp. 105028. Date of Electronic Publication: 2024 May 06.
DOI: 10.1016/j.jdent.2024.105028
Abstrakt: Aim: Three-dimensional (3D) cell culture systems perform better in resembling tissue or organism structures compared with traditional 2D models. Organs-on-chips (OoCs) are becoming more efficient 3D models. This study aimed to create a novel simplified dentin-on-a-chip using microfluidic chip technology and tissue engineering for screening dental materials.
Methodology: A microfluidic device with three channels was designed for creating 3D dental tissue constructs using stem cells from the apical papilla (SCAP) and gelatin methacrylate (GelMA). The study investigated the effect of varying cell densities and GelMA concentrations on the layer features formed within the microfluidic chip. Cell viability and distribution were evaluated through live/dead staining and nuclei/F-actin staining. The osteo/odontogenic potential was assessed through ALP staining and Alizarin red staining. The impact of GelMA concentrations (5 %, 10 %) on the osteo/odontogenic differentiation trajectory of SCAP was also studied.
Results: The 3D tissue constructs maintained high viability and favorable spreading within the microfluidic chip for 3-7 days. A cell seeding density of 2 × 10 4 cells/μL was found to be the most optimal choice, ensuring favorable cell proliferation and even distribution. GelMA concentrations of 5 % and 10 % proved to be most effective for promoting cell growth and uniform distribution. Within the 5 % GelMA group, SCAP demonstrated higher osteo/odontogenic differentiation than that in the 10 % GelMA group.
Conclusion: In 3D culture, GelMA concentration was found to regulate the osteo/odontogenic differentiation of SCAP. The study recommends a seeding density of 2 × 10 4 cells/μL of SCAP within 5 % GelMA for constructing simplified dentin-on-a-chip.
Clinical Significance: This study built up the 3D culture protocol, and induced odontogenic differentiation of SCAP, thus forming the simplified dentin-on-a-chip and paving the way to be used as a well-defined biological model for regenerative endodontics. It may serve as a potential testing platform for cell differentiation.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
Externí odkaz: