| Autor: |
Skaali R; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Christian Magnus Falsens vei 18, 1433, Ås, Norway. rannei.skaali@nmbu.no., Devle H; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Christian Magnus Falsens vei 18, 1433, Ås, Norway. rannei.skaali@nmbu.no., Ebner K; Bisy GmbH, Wünschendorf 292, 8200 Hofstätten an der Raab, Austria., Ekeberg D; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Christian Magnus Falsens vei 18, 1433, Ås, Norway. rannei.skaali@nmbu.no., Sørlie M; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Christian Magnus Falsens vei 18, 1433, Ås, Norway. rannei.skaali@nmbu.no. |
| Abstrakt: |
We have successfully developed a validated high-throughput analysis method for the identification and quantification of native and oxifunctionalized monolignols using direct infusion electrospray ionization tandem mass spectrometry (DI-ESI-MS/MS). Oxifunctionalized monolignols generated through unspecific peroxygenase catalysis present a sustainable alternative to fossil aromatic hydrocarbons. This study emphasizes a sustainable analytical approach for these renewable biocatalytic precursors, addressing challenges such as matrix effects, accuracy, precision, and sensitivity of the method. Our findings demonstrate the potential of overcoming quantification difficulties using DI-ESI-MS. Notably, this analytical methodology represents a novel utilization of DI-ESI-MS/MS in examining monolignols and their functionalization, thereby advancing the exploration of lignin as a valuable and sustainable bioresource. |
