Analysis of free, unbound thyroid hormones by liquid chromatography-tandem mass spectrometry: A mini-review of the medical rationale and analytical methods.

Autor: Westbye AB; Hormone Laboratory, Department of Medical Biochemistry Oslo University Hospital Oslo Norway.; Biochemical Endocrinology and Metabolism Research Group Oslo University Hospital Oslo Norway., Aas FE; Hormone Laboratory, Department of Medical Biochemistry Oslo University Hospital Oslo Norway.; Biochemical Endocrinology and Metabolism Research Group Oslo University Hospital Oslo Norway., Kelp O; Hormone Laboratory, Department of Medical Biochemistry Oslo University Hospital Oslo Norway.; Biochemical Endocrinology and Metabolism Research Group Oslo University Hospital Oslo Norway., Dahll LK; Hormone Laboratory, Department of Medical Biochemistry Oslo University Hospital Oslo Norway.; Biochemical Endocrinology and Metabolism Research Group Oslo University Hospital Oslo Norway., Thorsby PM; Hormone Laboratory, Department of Medical Biochemistry Oslo University Hospital Oslo Norway.; Biochemical Endocrinology and Metabolism Research Group Oslo University Hospital Oslo Norway.; Institute of Clinical Medicine University of Oslo Oslo Norway.
Jazyk: angličtina
Zdroj: Analytical science advances [Anal Sci Adv] 2023 Aug 02; Vol. 4 (7-8), pp. 244-254. Date of Electronic Publication: 2023 Aug 02 (Print Publication: 2023).
DOI: 10.1002/ansa.202200067
Abstrakt: Measurement of hormones is important for the diagnosis and management of endocrine diseases. The thyroid hormones thyroxine (T4) and triiodothyronine (T3) are among the most commonly measured hormones in clinical laboratories, and it is the concentration of free (not bound to proteins) thyroid hormones that is clinically most relevant. Free thyroid hormones are commonly measured using automated immunoassays, however, these are known to produce erroneous results due to interferences for some patients. Measurement of free thyroid hormones using equilibrium dialysis or ultrafiltration combined with liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is considered a more accurate and robust method for free thyroid hormone analysis and overcomes many of the limitations of immunoassays. However, LC-MS/MS-based methods are often considered too technically difficult and not amendable to high throughput by clinical chemists and are not offered by many clinical laboratories. This mini-review aims to make it easier for clinical laboratories to implement LC-MS/MS-based measurement of free thyroid hormones. It describes the medical rationale for measuring free thyroid hormones, the benefits of LC-MS/MS-based methods with respect to interferences affecting immunoassay-based methods and physical separation methods. This mini-review highlights important parameters for ultrafiltration and equilibrium dialysis to obtain physiologically relevant free thyroid hormone concentrations and focuses on methods and devices used in clinical chemistry.
Competing Interests: The authors declare no conflicts of interest.
(© 2023 The Authors. Analytical Science Advances published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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