Biochemical characterization of Bombyx mori Dicer-2 that dices double-stranded RNAs into 20-nt small RNA.

Autor: Tabara M; Ritsumeikan-Global Innovation Research Organization, Ritsumeikan University, Shiga, Japan., Harada M; Department of Biological Production, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan., Kuriyama K; Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan., Sakamoto T; Department of Biological Production, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan., Takeda A; Ritsumeikan-Global Innovation Research Organization, Ritsumeikan University, Shiga, Japan., Fukuhara T; Department of Applied Biological Sciences, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.; Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan., Tabunoki H; Department of Biological Production, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.; Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
Jazyk: angličtina
Zdroj: Archives of insect biochemistry and physiology [Arch Insect Biochem Physiol] 2024 May; Vol. 116 (1), pp. e22118.
DOI: 10.1002/arch.22118
Abstrakt: We detected enzymatic activity that generates 20-nucleotide (nt) RNA from double-stranded RNAs (dsRNAs) in crude extracts prepared from various silkworm (Bombyx mori) organs. The result using knocked-down cultured cells indicated that this dicing activity originated from B. mori Dicer-2 (BmDcr2). Biochemical analyses revealed that BmDcr2 preferentially cleaves 5'-phosphorylated dsRNAs at the 20-nt site-counted from the 5'-phosphorylated end-and required ATP and magnesium ions for the dicing reaction. This is the first report of the biochemical characterization of Dicer-2 in lepidopteran insects. This enzymatic property of BmDcr2 in vitro is consistent with the in vivo small interfering RNA profile in virus-infected silkworm cells.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
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