First molecular detection of Borrelia sp. in tapirs (Tapirus terrestris).

Autor: Mongruel ACB; Vector-Borne Bioagents Laboratory (VBBL), Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP) - Jaboticabal, São Paulo, Brazil., Medici EP; Iniciativa Nacional para Conservação da Anta Brasileira (INCAB), Instituto de Pesquisas Ecológicas (IPÊ) , Campo Grande, Mato Grosso do Sul, Brazil.; Escola Superior de Conservação Ambiental e Sustentabilidade (ESCAS/IPÊ) - Nazaré Paulista, São Paulo, Brazil.; Tapir Specialist Group (TSG), International Union for Conservation of Nature (IUCN SSC), Campo Grande, Mato Grosso do Sul, Brazil., da Costa Canena A; Iniciativa Nacional para Conservação da Anta Brasileira (INCAB), Instituto de Pesquisas Ecológicas (IPÊ) , Campo Grande, Mato Grosso do Sul, Brazil., Machado RZ; Vector-Borne Bioagents Laboratory (VBBL), Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP) - Jaboticabal, São Paulo, Brazil., Clay K; Department of Ecology and Evolutionary Biology, School of Sciences and Engineering, Tulane University, New Orleans, LA, USA., Labruna MB; Departamento de Medicina Veterinária Preventiva e Saúde Animal (VPS), Faculdade de Ciências Veterinárias e Zootecnia, Universidade de São Paulo (USP), São Paulo, São Paulo, Brazil., André MR; Vector-Borne Bioagents Laboratory (VBBL), Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP) - Jaboticabal, São Paulo, Brazil. mr.andre@unesp.br.
Jazyk: angličtina
Zdroj: Veterinary research communications [Vet Res Commun] 2024 Aug; Vol. 48 (4), pp. 2767-2774. Date of Electronic Publication: 2024 May 07.
DOI: 10.1007/s11259-024-10406-z
Abstrakt: Borrelia theileri is a tick-borne spirochete causative agent of fever, apathy and reduced food consumption in cattle. Molecular diagnosis has expanded the understanding of Borrelia theileri with new hosts and geographical locations being described. The present study aimed to describe the first molecular detection of B. theileri in wild tapirs (Tapirus terrestris) from South America. Blood DNA samples obtained from 99 tapirs sampled in Pantanal (n = 61) and Cerrado (n = 38) biomes were screened using a qPCR assay based on the 16 S rRNA gene of Borrelia sp. Positive samples in the qPCR assay were subjected to PCR assays to allow characterization of fragments from 16 S rRNA and flaB genes. Two (2/99; 2.0%) animals from Pantanal biome were positive in the qPCR and one sample presented bands of expected size for the flaB protocol. Amplicons from this sample were successfully cloned and sequenced. In the phylogenetic analysis, Borrelia sp. from T. terrestris grouped together with B. theileri sequences previously detected in Rhipicephalus microplus ticks and cattle from Minas Gerais State in Brazil, Rhipicephalus geigyi from Mali, and R. microplus and Haemaphysalis sulcata from Pakistan. This finding contributes to our knowledge regarding susceptible hosts species for B. theileri. More studies are necessary to understand the potential effects of B. theileri on tapir's health.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
Externí odkaz: