| Autor: |
Kim S; Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea., Heo J; Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea., Choi H; Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea.; Department of Biotechnology, Jeonbuk National University, Iksan 55496, Republic of Korea., Lee D; Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea., Kwon SW; Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea., Kim Y; Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea. |
| Abstrakt: |
An aerobic, Gram-stain-negative and short rod-shaped bacterial strain, designated M6-31 T , was isolated from rice paddy soil sampled in Miryang, Republic of Korea. Growth was observed at 4-35 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-4 % (w/v) NaCl (optimum, 0 % w/v). Phylogenetic analysis based on 16S rRNA gene sequences grouped strain M6-31 T with Sphingobacterium bambusae IBFC2009 T , Sphingobacterium griseoflavum SCU-B140 T and Sphingobacterium solani MLS-26-JM13-11 T in the same clade, with the 16S rRNA gene sequence similarities ranging from 95.8 to 96.6 %. A genome-based phylogenetic tree reconstructed by using all publicly available Sphingobacterium genomes placed strain M6-31 T with S. bambusae KACC 22910 T , ' Sphingobacterium deserti ' ACCC 05744 T , S. griseoflavum CGMCC 1.12966 T and Sphingobacterium paludis CGMCC 1.12801 T . Orthologous average nucleotide identity and digital DNA-DNA hybridization values between strain M6-31 T and its closely related strains were lower than 74.6 and 22.0 %, respectively. The respiratory quinone was menaquinone-7, and the major polar lipid was phosphatidylethanolamine. The major fatty acids (>10 %) were C 15 : 0 iso, C 17 : 0 iso 3OH and summed feature 3. The phenotypic, chemotaxonomic and genotypic data obtained in this study showed that strain M6-31 T represents a novel species of the genus Sphingobacterium , for which the name Sphingobacterium oryzagri sp. nov. (type strain M6-31 T =KACC 22765 T =JCM 35893 T ) is proposed. |
