First detection of triazole-resistant aspergillus fumigatus harbouring the TR34/L98H Cyp51A mutation in Burkina Faso.

Autor: Yerbanga IW; Centre Hospitalier Universitaire Régional de Ouahigouya, Ouahigouya, Burkina Faso.; Université Nazi Boni, Bobo-Dioulasso, Burkina Faso., Lagrou K; Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium.; Department of Laboratory Medicine and National Reference Center for Mycosis, Excellence Center for Medical Mycology (ECMM), University Hospitals Leuven, Leuven, Belgium., Merckx R; Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium.; Department of Laboratory Medicine and National Reference Center for Mycosis, Excellence Center for Medical Mycology (ECMM), University Hospitals Leuven, Leuven, Belgium., Nakanabo Diallo S; Université Nazi Boni, Bobo-Dioulasso, Burkina Faso.; Centre Muraz/Institut National de Santé Publique, Bobo-Dioulasso, Burkina Faso., Gangneux JP; Univ Rennes, CHU Rennes, Inserm, Irset (Institut de recherche en santé, environnement et travail), European ECMM Excellence Center in Medical Mycology, Rennes, France., Delabarre A; Univ Rennes, CHU Rennes, Inserm, Irset (Institut de recherche en santé, environnement et travail), European ECMM Excellence Center in Medical Mycology, Rennes, France., Denis O; Department of Microbiology, CHU Namur site-Godinne, Université Catholique de Louvain, Brussels, Belgium.; Ecole de Santé Publique, Université Libre de Bruxelles, Brussels, Belgium., Rodriguez-Villalobos H; Department of Microbiology, Cliniques Universitaires Saint-Luc-Université Catholique de Louvain, Brussels, Belgium., Montesinos I; Department of Microbiology, CHU Namur site-Godinne, Université Catholique de Louvain, Brussels, Belgium., Bamba S; Université Nazi Boni, Bobo-Dioulasso, Burkina Faso.; Centre Hospitalier Universitaire Sourô Sanou, Bobo-Dioulasso, Burkina Faso.
Jazyk: angličtina
Zdroj: Mycoses [Mycoses] 2024 May; Vol. 67 (5), pp. e13732.
DOI: 10.1111/myc.13732
Abstrakt: Background: Triazole-resistant Aspergillus fumigatus (TRAF) isolates are a growing public health problem with worldwide distribution. Epidemiological data on TRAF is limited in Africa, particularly in West Africa.
Objectives: This study aimed to screen for the environmental presence of TRAF isolates in the indoor air of two hospitals in Burkina Faso.
Materials and Methods: Air samples were collected in wards housing patients at risk for invasive aspergillosis, namely infectious diseases ward, internal medicine ward, nephrology ward, pulmonology ward, medical emergency ward and paediatric ward. Sabouraud Dextrose Agar supplemented with triazoles was used to screen the suspected TRAF isolates and EUCAST method to confirm the resistance of suspected isolates. Sequencing of cyp51A gene was used to identify the resistance mechanism of confirmed TRAF isolates.
Results: Of the 198 samples collected and analysed, 67 showed growth of A. fumigatus isolates. The prevalence of TRAF isolates was 3.23% (4/124). One TRAF isolate exhibited a pan-triazole resistance. Sequencing of cyp51A gene identified the TR34/L98H mutation for this pan-triazole resistant isolate. This study showed for the first time the circulation of the pan-azole resistant isolate harbouring the TR34/L98H mutation in Burkina Faso.
Conclusions: These findings emphasise the need to map these TRAF isolates in all parts of Burkina Faso and to establish local and national continuous surveillance of environmental and clinical TRAF isolates in this country.
(© 2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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