Morphometric and histopathological evaluation of modified Elnady's plastinated tissue compared to non-plastinated tissue: Highlighting its relevance for teaching and research.

Autor: Ahmed O; Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt., Gaballa MMS; Department of Pathology, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt., Abumandour MMA; Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt., Al-Otaibi AM; Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia., Choudhary P; Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Rampura Phul, Punjab, India., El-Shafey AA; Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt.; Department of Basic veterinary sciences, Faculty of Veterinary Medicine, Delta University for Science and Technology, Dakahlia, Egypt.
Jazyk: angličtina
Zdroj: Anatomia, histologia, embryologia [Anat Histol Embryol] 2024 May; Vol. 53 (3), pp. e13046.
DOI: 10.1111/ahe.13046
Abstrakt: The present study aims to evaluate the morphometric and histopathological properties of Modified Elnady's plastinated tissue after a period compared to non-plastinated tissue. The plastination technique is utilized in research and teaching due to the potential health risks associated with prolonged exposure to formalin. The tissues and organs are permanently dried during plastination and can be used for further anatomical, histopathological and surgical educational purposes. This method involves drying tissue and allowing synthetic materials like glycerin to permeate it. The study compared non-plastinated and plastinated tissue post-plastination to determine if structural alterations differed from those linked to plastination. The study examined the histopathological examination of dogs' skin, muscles, liver, lung, and intestine using formalin-fixed organs for paraffin embedding and previously plastinated organs for a plastinated group. The study examined non-plastinated and plastinated tissues, their histological composition and biometric parameters revealing typical structures in the non-plastinated group. Plasmodiumted tissues exhibited a compacted appearance, volume changes, nuclear clarity, and cytoplasmic hypereosinophilia, with statistical differences between the two groups. The study reveals that plastinated tissues, after 5 years of plastination, maintain their histological architecture well, with some exceptions. Plastinated tissues can be utilized in future microscopic and immunological studies and will be beneficial for teaching and research.
(© 2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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