Two mosquito salivary antigens demonstrate promise as biomarkers of recent exposure to P. falciparum infected mosquito bites.

Autor:	Lapidus S; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA., Goheen MM; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.; Division of Infectious Diseases, Yale School of Medicine, New Haven, CT, USA., Sy M; Laboratory of Parasitology and Mycology, Cheikh Anta Diop University, Aristide le Dantec Hospital, Dakar, Senegal.; International Research and Training Center for Applied Genomics and Health Surveillance (CIGASS) at UCAD, Dakar, Senegal., Deme AB; Laboratory of Parasitology and Mycology, Cheikh Anta Diop University, Aristide le Dantec Hospital, Dakar, Senegal.; International Research and Training Center for Applied Genomics and Health Surveillance (CIGASS) at UCAD, Dakar, Senegal., Ndiaye IM; Laboratory of Parasitology and Mycology, Cheikh Anta Diop University, Aristide le Dantec Hospital, Dakar, Senegal.; International Research and Training Center for Applied Genomics and Health Surveillance (CIGASS) at UCAD, Dakar, Senegal., Diedhiou Y; Laboratory of Parasitology and Mycology, Cheikh Anta Diop University, Aristide le Dantec Hospital, Dakar, Senegal.; International Research and Training Center for Applied Genomics and Health Surveillance (CIGASS) at UCAD, Dakar, Senegal., Mbaye AM; Laboratory of Parasitology and Mycology, Cheikh Anta Diop University, Aristide le Dantec Hospital, Dakar, Senegal.; International Research and Training Center for Applied Genomics and Health Surveillance (CIGASS) at UCAD, Dakar, Senegal., Hagadorn KA; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA., Sene SD; G4 - Malaria Experimental Genetic Approaches & Vaccines, Pôle Immunophysiopathologie et Maladies Infectieuses, Institut Pasteur de Dakar, Dakar, Senegal., Pouye MN; G4 - Malaria Experimental Genetic Approaches & Vaccines, Pôle Immunophysiopathologie et Maladies Infectieuses, Institut Pasteur de Dakar, Dakar, Senegal., Thiam LG; G4 - Malaria Experimental Genetic Approaches & Vaccines, Pôle Immunophysiopathologie et Maladies Infectieuses, Institut Pasteur de Dakar, Dakar, Senegal., Ba A; G4 - Malaria Experimental Genetic Approaches & Vaccines, Pôle Immunophysiopathologie et Maladies Infectieuses, Institut Pasteur de Dakar, Dakar, Senegal., Guerra N; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA., Mbengue A; G4 - Malaria Experimental Genetic Approaches & Vaccines, Pôle Immunophysiopathologie et Maladies Infectieuses, Institut Pasteur de Dakar, Dakar, Senegal., Raduwan H; Division of Infectious Diseases, Yale School of Medicine, New Haven, CT, USA., Vigan-Womas I; G4 - Malaria Experimental Genetic Approaches & Vaccines, Pôle Immunophysiopathologie et Maladies Infectieuses, Institut Pasteur de Dakar, Dakar, Senegal., Parikh S; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.; Division of Infectious Diseases, Yale School of Medicine, New Haven, CT, USA., Ko AI; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.; Division of Infectious Diseases, Yale School of Medicine, New Haven, CT, USA., Ndiaye D; Laboratory of Parasitology and Mycology, Cheikh Anta Diop University, Aristide le Dantec Hospital, Dakar, Senegal.; International Research and Training Center for Applied Genomics and Health Surveillance (CIGASS) at UCAD, Dakar, Senegal., Fikrig E; Division of Infectious Diseases, Yale School of Medicine, New Haven, CT, USA., Chuang YM; Division of Infectious Diseases, Yale School of Medicine, New Haven, CT, USA., Bei AK; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.; Laboratory of Parasitology and Mycology, Cheikh Anta Diop University, Aristide le Dantec Hospital, Dakar, Senegal.; G4 - Malaria Experimental Genetic Approaches & Vaccines, Pôle Immunophysiopathologie et Maladies Infectieuses, Institut Pasteur de Dakar, Dakar, Senegal.
Jazyk:	angličtina
Zdroj:	MedRxiv : the preprint server for health sciences [medRxiv] 2024 Apr 22. Date of Electronic Publication: 2024 Apr 22.
DOI:	10.1101/2024.04.20.24305430
Abstrakt:	Background: Measuring malaria transmission intensity using the traditional entomological inoculation rate is difficult. Antibody responses to mosquito salivary proteins such as SG6 have previously been used as biomarkers of exposure to Anopheles mosquito bites. Here, we investigate four mosquito salivary proteins as potential biomarkers of human exposure to mosquitoes infected with P. falciparum : mosGILT, SAMSP1, AgSAP, and AgTRIO. Methods: We tested population-level human immune responses in longitudinal and cross-sectional plasma samples from individuals with known P. falciparum infection from low and moderate transmission areas in Senegal using a multiplexed magnetic bead-based assay. Results: AgSAP and AgTRIO were the best indicators of recent exposure to infected mosquitoes. Antibody responses to AgSAP, in a moderate endemic area, and to AgTRIO in both low and moderate endemic areas, were significantly higher than responses in a healthy non-endemic control cohort (p-values = 0.0245, 0.0064, and <0.0001 respectively). No antibody responses significantly differed between the low and moderate transmission area, or between equivalent groups during and outside the malaria transmission seasons. For AgSAP and AgTRIO, reactivity peaked 2-4 weeks after clinical P. falciparum infection and declined 3 months after infection. Discussion: Reactivity to both AgSAP and AgTRIO peaked after infection and did not differ seasonally nor between areas of low and moderate transmission, suggesting reactivity is likely reflective of exposure to infectious mosquitos or recent biting rather than general mosquito exposure. Kinetics suggest reactivity is relatively short-lived. AgSAP and AgTRIO are promising candidates to incorporate into multiplexed assays for serosurveillance of population-level changes in P. falciparum -infected mosquito exposure.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu