Assessing the virucidal activity of essential oils against feline calicivirus, a non-enveloped virus used as surrogate of norovirus.

Autor: Lanave G; Department of Veterinary Medicine, University of Bari Aldo Moro, 70010, Valenzano, Bari, Italy., Catella C; Department of Veterinary Medicine, University of Bari Aldo Moro, 70010, Valenzano, Bari, Italy., Catalano A; Department of Pharmacy-Drug Sciences, University of Bari Aldo Moro, 70125, Bari, Italy., Lucente MS; Department of Veterinary Medicine, University of Bari Aldo Moro, 70010, Valenzano, Bari, Italy., Pellegrini F; Department of Veterinary Medicine, University of Bari Aldo Moro, 70010, Valenzano, Bari, Italy., Fracchiolla G; Department of Pharmacy-Drug Sciences, University of Bari Aldo Moro, 70125, Bari, Italy., Diakoudi G; Department of Veterinary Medicine, University of Bari Aldo Moro, 70010, Valenzano, Bari, Italy., Palmisani J; Department of Biosciences, Biotechnologies and Environment, University of Bari Aldo Moro, 70126, Bari, Italy., Trombetta CM; Department of Molecular and Developmental Medicine, University of Siena, 53100, Siena, Italy., Martella V; Department of Veterinary Medicine, University of Bari Aldo Moro, 70010, Valenzano, Bari, Italy., Camero M; Department of Veterinary Medicine, University of Bari Aldo Moro, 70010, Valenzano, Bari, Italy.
Jazyk: angličtina
Zdroj: Heliyon [Heliyon] 2024 Apr 29; Vol. 10 (9), pp. e30492. Date of Electronic Publication: 2024 Apr 29 (Print Publication: 2024).
DOI: 10.1016/j.heliyon.2024.e30492
Abstrakt: Norovirus (NoV) causes serious gastrointestinal disease worldwide and is regarded as an important foodborne pathogen. Due the difficulties of in vitro cultivation for human NoV, alternative caliciviruses (i.e., feline calicivirus, FCV, or murine NoV) have long been used as surrogates for in vitro assessment of the efficacy of antivirals. Essential oils (EOs) are natural compounds that have displayed antimicrobial and antioxidant properties. We report in vitro the virucidal efficacy of four EOs, Melissa officinalis L. EO (MEO), Thymus vulgaris L. EO (TEO), Rosmarinus officinalis L. EO (REO), and Salvia officinalis L. EO (SEO) against FCV at different time contacts (10, 30 min, 1, 4 and 8 h). At the maximum non-cytotoxic concentration and at 10- and 100- fold concentrations over the cytotoxic threshold, the EOs did not decrease significantly FCV viral titers. However, MEO at 12,302.70 μg/mL exhibited a significant efficacy decreasing the viral titer by 0.75 log 10 Tissue Culture Infectious Dose (TCID 50 )/50 μl after 10 min as compared to virus control. In this study, virucidal activity of four EOs against FCV, was investigated. A lack of virucidal efficacy of TEO, REO and SEO at different compound concentrations and time contacts against FCV was observed whilst MEO was able to significantly decrease FCV titer.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2024 The Authors.)
Databáze: MEDLINE
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