The expression of salivary EGF, VEGF, endothelin, and transferrin in waterpipe and cigarette smokers.

Autor: Alrashdan MS; Department of Oral and Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, P.O.Box: 27272, Sharjah, UAE. malrashdan@sharjah.ac.ae.; Department of Oral Medicine and Oral Surgery, Faculty of Dentistry, Jorda University of Science and Technology, Irbid, Jordan. malrashdan@sharjah.ac.ae., Al-Shorman H; Department of Oral and Maxillofacial Surgery and Periodontics, Faculty of Dentistry, Zarqa University, Zarqa, Jordan.; Department of Preventive Dentistry, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan., Bouzid A; Research Institute for Medical and Health Sciences, University of Sharjah, Sharjah, UAE., Al-Dwairi A; Department of Physiology and Biochemistry, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan., Alazzam M; Department of Oral Medicine and Oral Surgery, Faculty of Dentistry, Jorda University of Science and Technology, Irbid, Jordan., Alqudah M; Physiology Department, Arabian Gulf University, Manama, Bahrain.
Jazyk: angličtina
Zdroj: Odontology [Odontology] 2024 May 06. Date of Electronic Publication: 2024 May 06.
DOI: 10.1007/s10266-024-00947-9
Abstrakt: The aim of this study was to evaluate the effects of two forms of tobacco smoking, cigarettes and water pipe smoking (WPS), on the expression of a panel of salivary proteins in healthy adults. Three groups of age and gender-matched participants were enrolled in the study: never-smokers, cigarette smokers and WPS (N = 55 per group). Expression of epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), endothelin and transferrin in unstimulated whole saliva was estimated using enzyme-linked immunosorbent assays. Statistical analysis consisted of one-way ANOVA and Tukey's post hoc tests, in addition to bioinformatics analysis. VEGF expression was the least in WPS (51.1 ± 14.5 pg/ml) compared to both controls (150.1 ± 13.8 pg/ml) and cigarette smokers (93 ± 9.9 pg/ml), with a significant difference in WPS (p < 0.001) and cigarette smokers (p < 0.01) compared to controls. Furthermore, transferrin showed the weakest expression in the WPS group (1238 ± 261.4 pg/ml) compared to controls (2205.6 ± 298.6 pg/ml) (p = 0.05) and cigarette smokers (1805.4 ± 244 pg/ml). Neither EGF nor endothelin expression showed any statistical difference between the groups (p > 0.05). Gene-gene interaction network demonstrated that FLT1, TFRC, KDR, VEGFB and PGF genes had the highest potential for interaction with the studied proteins. Further functional annotations on the identified markers in the interaction network were performed to identify HIF-1 pathways among the most relevant pathways. In conclusion, smoking habits alter the expression of salivary VEGF and transferrin, which may correspond to early sub-clinical changes in the oral mucosa. The clinical relevance of these salivary changes requires further research.
(© 2024. The Author(s), under exclusive licence to The Society of The Nippon Dental University.)
Databáze: MEDLINE
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