Synthetic phage-based approach for sensitive and specific detection of Escherichia coli O157.

Autor: Tamura A; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.; Division of Infectious Diseases, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan., Azam AH; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan., Nakamura T; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan., Lee K; Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan., Iyoda S; Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan., Kondo K; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan., Ojima S; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan., Chihara K; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan., Yamashita W; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.; Department of Life Science and Medical Bioscience, Waseda University, Shinjuku-ku, Tokyo, Japan., Cui L; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke-shi, Tochigi, Japan., Akeda Y; Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan., Watashi K; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan., Takahashi Y; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.; Department of Life Science and Medical Bioscience, Waseda University, Shinjuku-ku, Tokyo, Japan., Yotsuyanagi H; Division of Infectious Diseases, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan., Kiga K; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan. k-kiga@niid.go.jp.; Division of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University, Shimotsuke-shi, Tochigi, Japan. k-kiga@niid.go.jp.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2024 May 06; Vol. 7 (1), pp. 535. Date of Electronic Publication: 2024 May 06.
DOI: 10.1038/s42003-024-06247-w
Abstrakt: Escherichia coli O157 can cause foodborne outbreaks, with infection leading to severe disease such as hemolytic-uremic syndrome. Although phage-based detection methods for E. coli O157 are being explored, research on their specificity with clinical isolates is lacking. Here, we describe an in vitro assembly-based synthesis of vB_Eco4M-7, an O157 antigen-specific phage with a 68-kb genome, and its use as a proof of concept for E. coli O157 detection. Linking the detection tag to the C-terminus of the tail fiber protein, gp27 produces the greatest detection sensitivity of the 20 insertions sites tested. The constructed phage detects all 53 diverse clinical isolates of E. coli O157, clearly distinguishing them from 35 clinical isolates of non-O157 Shiga toxin-producing E. coli. Our efficient phage synthesis methods can be applied to other pathogenic bacteria for a variety of applications, including phage-based detection and phage therapy.
(© 2024. The Author(s).)
Databáze: MEDLINE
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