Detection of clonal plasma cells in POEMS syndrome using multiparameter flow cytometry.

Autor: Ishii A; Department of Hematology, Chiba University Hospital, Chiba, Japan.; Department of Endocrinology, Hematology, and Gerontology, Chiba University Graduate School of Medicine, Chiba, Japan., Tsukamoto S; Department of Hematology, Chiba University Hospital, Chiba, Japan. shokichi.tsukamoto@chiba-u.jp.; Department of Endocrinology, Hematology, and Gerontology, Chiba University Graduate School of Medicine, Chiba, Japan. shokichi.tsukamoto@chiba-u.jp., Mimura N; Department of Hematology, Chiba University Hospital, Chiba, Japan.; Department of Transfusion Medicine and Cell Therapy, Chiba University Hospital, Chiba, Japan., Miyamoto-Nagai Y; Department of Hematology, Chiba University Hospital, Chiba, Japan., Isshiki Y; Department of Hematology, Chiba University Hospital, Chiba, Japan., Matsui S; Department of Hematology, Chiba University Hospital, Chiba, Japan., Nakao S; Department of Hematology, Chiba University Hospital, Chiba, Japan., Shibamiya A; Department of Hematology, Chiba University Hospital, Chiba, Japan., Hino Y; Department of Hematology, Chiba University Hospital, Chiba, Japan., Kayamori K; Department of Hematology, Chiba University Hospital, Chiba, Japan., Oshima-Hasegawa N; Department of Hematology, Chiba University Hospital, Chiba, Japan., Muto T; Department of Hematology, Chiba University Hospital, Chiba, Japan., Takeda Y; Department of Hematology, Chiba University Hospital, Chiba, Japan., Suichi T; Department of Neurology, Chiba University Graduate School of Medicine, Chiba, Japan., Misawa S; Department of Neurology, Chiba University Graduate School of Medicine, Chiba, Japan., Ohwada C; Department of Hematology, Chiba University Hospital, Chiba, Japan.; Department of Hematology, International University of Health and Welfare, Narita, Japan., Yokote K; Department of Endocrinology, Hematology, and Gerontology, Chiba University Graduate School of Medicine, Chiba, Japan., Kuwabara S; Department of Neurology, Chiba University Graduate School of Medicine, Chiba, Japan., Nakaseko C; Department of Hematology, International University of Health and Welfare, Narita, Japan., Takamatsu H; Department of Hematology, Institute of Medical, Pharmaceutical and Health Sciences, Faculty of Medicine, Kanazawa University, Kanazawa, Japan., Sakaida E; Department of Hematology, Chiba University Hospital, Chiba, Japan.; Department of Endocrinology, Hematology, and Gerontology, Chiba University Graduate School of Medicine, Chiba, Japan.; Department of Transfusion Medicine and Cell Therapy, Chiba University Hospital, Chiba, Japan.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 May 06; Vol. 14 (1), pp. 10362. Date of Electronic Publication: 2024 May 06.
DOI: 10.1038/s41598-024-61034-1
Abstrakt: POEMS syndrome (polyneuropathy, organomegaly, endocrinopathy, monoclonal protein [M-protein], and skin changes) is a rare systemic disorder characterized by various symptoms caused by underlying plasma cell (PC) dyscrasia. Detection of monoclonal PCs is mandatory for the diagnosis of POEMS syndrome; however, the usefulness of EuroFlow-based next-generation flow cytometry (EuroFlow-NGF) in POEMS syndrome for detecting monoclonal PCs in bone marrow (BM) and the gating strategy suitable for flow cytometry study of POEMS syndrome remain unknown. We employed EuroFlow-NGF-based single-tube eight-color multiparameter flow cytometry (MM-flow) and established a new gating strategy (POEMS-flow) to detect the monoclonal PCs in POEMS syndrome, gating CD38 broadly from dim to bright and CD45 narrowly from negative to dim compared to MM-flow. MM-flow detected monoclonal PCs in 9/25 (36.0%) cases, including 2/2 immunofixation electrophoresis (IFE)-negative cases (100%). However, POEMS-flow detected monoclonal PCs in 18/25 cases (72.0%), including 2/2 IFE-negative cases (100%). POEMS-flow detected monoclonal PCs with immunophenotypes of CD19 - in 17/18 (94.4%). In six cases where post-treatment samples were available, the size of the clones was significantly reduced after the treatment (P = 0.031). POEMS-flow can enhance the identification rate of monoclonal PCs in POEMS syndrome and become a valuable tool for the diagnosis of POEMS syndrome.
(© 2024. The Author(s).)
Databáze: MEDLINE
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