Modularized Engineering of Shewanella oneidensis MR-1 for Efficient and Directional Synthesis of 5-Aminolevulinic Acid.

Autor: Wu J; CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science & Technology of China, Hefei, 230026, China; Institute of Advanced Technology, University of Science and Technology of China, Hefei, 230000, China., Wu J; School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, 230026, China., He RL; CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science & Technology of China, Hefei, 230026, China; Institute of Advanced Technology, University of Science and Technology of China, Hefei, 230000, China., Hu L; School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, 230026, China., Liu DF; CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science & Technology of China, Hefei, 230026, China. Electronic address: dfl@ustc.edu.cn., Li WW; CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science & Technology of China, Hefei, 230026, China; Institute of Advanced Technology, University of Science and Technology of China, Hefei, 230000, China; School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, 230026, China. Electronic address: wwli@ustc.edu.cn.
Jazyk: angličtina
Zdroj: Metabolic engineering [Metab Eng] 2024 May; Vol. 83, pp. 206-215. Date of Electronic Publication: 2024 May 06.
DOI: 10.1016/j.ymben.2024.05.001
Abstrakt: Shewanella oneidensis MR-1 has found widespread applications in pollutant transformation and bioenergy production, closely tied to its outstanding heme synthesis capabilities. However, this significant biosynthetic potential is still unexploited so far. Here, we turned this bacterium into a highly-efficient bio-factory for green synthesis of 5-Aminolevulinic Acid (5-ALA), an important chemical for broad applications in agriculture, medicine, and the food industries. The native C5 pathway genes of S. oneidensis was employed, together with the introduction of foreign anti-oxidation module, to establish the 5-ALA production module, resulting 87-fold higher 5-ALA yield and drastically enhanced tolerance than the wild type. Furthermore, the metabolic flux was regulated by using CRISPR interference and base editing techniques to suppress the competitive pathways to further improve the 5-ALA titer. The engineered strain exhibited 123-fold higher 5-ALA production capability than the wild type. This study not only provides an appealing new route for 5-ALA biosynthesis, but also presents a multi-dimensional modularized engineering strategy to broaden the application scope of S. oneidensis.
(Copyright © 2024 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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