Structural basis for raccoon dog receptor recognition by SARS-CoV-2.

Autor: Hsueh FC; Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America.; Center for Emerging Viruses, University of Minnesota, Minneapolis, Minnesota, United States of America., Shi K; Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, United States of America., Mendoza A; Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America.; Center for Emerging Viruses, University of Minnesota, Minneapolis, Minnesota, United States of America., Bu F; Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America.; Center for Emerging Viruses, University of Minnesota, Minneapolis, Minnesota, United States of America., Zhang W; Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America.; Center for Emerging Viruses, University of Minnesota, Minneapolis, Minnesota, United States of America., Aihara H; Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, United States of America., Li F; Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota, United States of America.; Center for Emerging Viruses, University of Minnesota, Minneapolis, Minnesota, United States of America.
Jazyk: angličtina
Zdroj: PLoS pathogens [PLoS Pathog] 2024 May 06; Vol. 20 (5), pp. e1012204. Date of Electronic Publication: 2024 May 06 (Print Publication: 2024).
DOI: 10.1371/journal.ppat.1012204
Abstrakt: Since the COVID-19 outbreak, raccoon dogs have been suggested as a potential intermediary in transmitting SARS-CoV-2 to humans. To understand their role in the COVID-19 pandemic and the species barrier for SARS-CoV-2 transmission to humans, we analyzed how their ACE2 protein interacts with SARS-CoV-2 spike protein. Biochemical data showed that raccoon dog ACE2 is an effective receptor for SARS-CoV-2 spike protein, though not as effective as human ACE2. Structural comparisons highlighted differences in the virus-binding residues of raccoon dog ACE2 compared to human ACE2 (L24Q, Y34H, E38D, T82M, R353K), explaining their varied effectiveness as receptors for SARS-CoV-2. These variations contribute to the species barrier that exists between raccoon dogs and humans regarding SARS-CoV-2 transmission. Identifying these barriers can help assess the susceptibility of other mammals to SARS-CoV-2. Our research underscores the potential of raccoon dogs as SARS-CoV-2 carriers and identifies molecular barriers that affect the virus's ability to jump between species.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Hsueh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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