Co-occurring microflora and mucin drive Pseudomonas aeruginosa diversification and pathoadaptation.

Autor:	Bottery MJ; Division of Evolution Infection and Genomics, School of Biological Sciences, University of Manchester, Manchester M13 9PL, United Kingdom., Johansen HK; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.; Department of Clinical Microbiology, Rigshospitalet, Copenhagen University Hospital, Copenhagen 9301, Denmark.; Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2200, Denmark., Pitchford JW; Department of Biology, University of York, Wentworth Way, York YO10 5DD, United Kingdom.; Department of Mathematics, University of York, Heslington, York YO10 5DD, United Kingdom., Friman VP; Department of Biology, University of York, Wentworth Way, York YO10 5DD, United Kingdom.; Department of Microbiology, University of Helsinki, Helsinki 00014, Finland.
Jazyk:	angličtina
Zdroj:	ISME communications [ISME Commun] 2024 Mar 28; Vol. 4 (1), pp. ycae043. Date of Electronic Publication: 2024 Mar 28 (Print Publication: 2024).
DOI:	10.1093/ismeco/ycae043
Abstrakt:	While several environmental factors contribute to the evolutionary diversification of the pathogenic bacterium Pseudomonas aeruginosa during cystic fibrosis lung infections, relatively little is known about the impact of the surrounding microbiota. By using in vitro experimental evolution , we show that the presence of Stenotrophomonas maltophilia , Staphylococcus aureus, or them both, prevent the evolution of loss of virulence, which repeatedly occurs in the absence of these species due to mutations in regulators of the Pseudomonas Quinolone Signal quorum sensing system, vqsM and pqsR . Moreover, the strength of the effect of co-occurring species is attenuated through changes in the physical environment by the addition of mucin, resulting in selection for phenotypes resembling those evolved in the absence of the co-occurring species. Together, our findings show that variation in mucosal environment and the surrounding polymicrobial environment can determine the evolutionary trajectory of P. aeruginosa , partly explaining its diversification and pathoadaptation from acute to chronic phenotype during cystic fibrosis lung infections. Competing Interests: The authors declared no conflict of interests. (© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu