High-fat diet, microbiome-gut-brain axis signaling, and anxiety-like behavior in male rats.

Autor: de Noronha SISR; Department of Biological Sciences, Laboratory of Cardiovascular Physiology, Federal University of Ouro Preto, Ouro Preto, MG, 35400-000, Brazil.; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, 80309, USA., de Moraes LAG; Department of Biological Sciences, Laboratory of Cardiovascular Physiology, Federal University of Ouro Preto, Ouro Preto, MG, 35400-000, Brazil.; Computing Department, Federal University of Ouro Preto, Ouro Preto, MG, 35400-000, Brazil., Hassell JE Jr; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, 80309, USA., Stamper CE; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, 80309, USA., Arnold MR; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, 80309, USA., Heinze JD; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, 80309, USA., Foxx CL; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, 80309, USA., Lieb MM; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, 80309, USA., Cler KE; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, 80309, USA., Karns BL; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, 80309, USA., Jaekel S; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, 80309, USA., Loupy KM; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, 80309, USA., Silva FCS; Department of Biological Sciences, Laboratory of Cardiovascular Physiology, Federal University of Ouro Preto, Ouro Preto, MG, 35400-000, Brazil., Chianca-Jr DA; Department of Biological Sciences, Laboratory of Cardiovascular Physiology, Federal University of Ouro Preto, Ouro Preto, MG, 35400-000, Brazil., Lowry CA; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, 80309, USA.; Department of Psychology and Neuroscience, University of Colorado Boulder, Boulder, CO, 80309, USA.; Center for Neuroscience, University of Colorado Boulder, Boulder, CO, 80309, USA., de Menezes RC; Department of Biological Science Laboratory of Cardiovascular Physiology, Campus Morro do Cruzeiro s/n, Ouro Preto, 35400-000, MG, Brazil. rodrigo.menezes@ufop.edu.br.
Jazyk: angličtina
Zdroj: Biological research [Biol Res] 2024 May 06; Vol. 57 (1), pp. 23. Date of Electronic Publication: 2024 May 06.
DOI: 10.1186/s40659-024-00505-1
Abstrakt: Obesity, associated with the intake of a high-fat diet (HFD), and anxiety are common among those living in modern urban societies. Recent studies suggest a role of microbiome-gut-brain axis signaling, including a role for brain serotonergic systems in the relationship between HFD and anxiety. Evidence suggests the gut microbiome and the serotonergic brain system together may play an important role in this response. Here we conducted a nine-week HFD protocol in male rats, followed by an analysis of the gut microbiome diversity and community composition, brainstem serotonergic gene expression (tph2, htr1a, and slc6a4), and anxiety-related defensive behavioral responses. We show that HFD intake decreased alpha diversity and altered the community composition of the gut microbiome in association with obesity, increased brainstem tph2, htr1a and slc6a4 mRNA expression, including in the caudal part of the dorsomedial dorsal raphe nucleus (cDRD), a subregion previously associated with stress- and anxiety-related behavioral responses, and, finally, increased anxiety-related defensive behavioral responses. The HFD increased the Firmicutes/Bacteroidetes ratio relative to control diet, as well as higher relative abundances of Blautia, and decreases in Prevotella. We found that tph2, htr1a and slc6a4 mRNA expression were increased in subregions of the dorsal raphe nucleus in the HFD, relative to control diet. Specific bacterial taxa were associated with increased serotonergic gene expression in the cDRD. Thus, we propose that HFD-induced obesity is associated with altered microbiome-gut-serotonergic brain axis signaling, leading to increased anxiety-related defensive behavioral responses in rats.
(© 2024. The Author(s).)
Databáze: MEDLINE