A Hitchhiker's guide to high-dimensional tissue imaging with multiplexed ion beam imaging.

Autor:	Yeo YY; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, United States; Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, MA, United States., Cramer P; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, United States., Deisher A; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, United States., Bai Y; Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA, United States., Zhu B; Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA, United States., Yeo WJ; Department of Physics, Institute of Learning and Brain Sciences, University of Washington, Seattle, WA, United States., Shipp MA; Department of Medical Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, United States., Rodig SJ; Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, United States., Jiang S; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, United States; Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, MA, United States; Department of Pathology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, United States; Broad Institute of MIT and Harvard, Cambridge, MA, United States. Electronic address: sjiang3@bidmc.harvard.edu.
Jazyk:	angličtina
Zdroj:	Methods in cell biology [Methods Cell Biol] 2024; Vol. 186, pp. 213-231. Date of Electronic Publication: 2024 Mar 10.
DOI:	10.1016/bs.mcb.2024.02.018
Abstrakt:	Advancements in multiplexed tissue imaging technologies are vital in shaping our understanding of tissue microenvironmental influences in disease contexts. These technologies now allow us to relate the phenotype of individual cells to their higher-order roles in tissue organization and function. Multiplexed Ion Beam Imaging (MIBI) is one of such technologies, which uses metal isotope-labeled antibodies and secondary ion mass spectrometry (SIMS) to image more than 40 protein markers simultaneously within a single tissue section. Here, we describe an optimized MIBI workflow for high-plex analysis of Formalin-Fixed Paraffin-Embedded (FFPE) tissues following antigen retrieval, metal isotope-conjugated antibody staining, imaging using the MIBI instrument, and subsequent data processing and analysis. While this workflow is focused on imaging human FFPE samples using the MIBI, this workflow can be easily extended to model systems, biological questions, and multiplexed imaging modalities. (Copyright © 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.)
Databáze:	MEDLINE
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