PLA 2 -MjTX-II from Bothrops moojeni snake venom exhibits antimetastatic and antiangiogenic effects on human lung cancer cells.

Autor: Santos LC; Institute Multidisciplinary in Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil., Oliveira VQ; Institute Multidisciplinary in Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil., Teixeira SC; Department of Immunology, Institute of Biomedical Sciences, Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil., Correia TML; Institute Multidisciplinary in Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil., Andrade LOSB; Institute Multidisciplinary in Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil., Polloni L; Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology, Federal University of Uberlândia, (UFU), Uberlândia, MG, Brazil., Marques LM; Institute Multidisciplinary in Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil., Clissa PB; Laboratory of Immunopathology, Butantan Institute, São Paulo, SP, Brazil., Baldo C; Department of Biochemistry and Biotechnology, State University of Londrina (UEL), Londrina, PR, Brazil., Ferro EAV; Department of Immunology, Institute of Biomedical Sciences, Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil., Gusmão ACMM; Institute Multidisciplinary in Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil., Silva MJB; Department of Immunology, Institute of Biomedical Sciences, Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil., Sanabani SS; Laboratory of Medical Investigation in Dermatology and Immunodeficiency, São Paulo Institute of Tropical Medicine, School of Medicine, University of São Paulo (USP), São Paulo, SP, Brazil., Ávila VMR; Laboratory of Biochemistry and Animal Toxins, Institute of Biotechnology, Federal University of Uberlândia, (UFU), Uberlândia, MG, Brazil. Electronic address: vmravila@hotmail.com., Lopes DS; Institute Multidisciplinary in Health, Federal University of Bahia (UFBA), Vitória da Conquista, BA, Brazil. Electronic address: lsdaiana@yahoo.com.br.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2024 May 28; Vol. 243, pp. 107742. Date of Electronic Publication: 2024 May 04.
DOI: 10.1016/j.toxicon.2024.107742
Abstrakt: Phospholipases A 2 (PLA 2 s) from snake venom possess antitumor and antiangiogenic properties. In this study, we evaluated the antimetastatic and antiangiogenic effects of MjTX-II, a Lys49 PLA 2 isolated from Bothrops moojeni venom, on lung cancer and endothelial cells. Using in vitro and ex vivo approaches, we demonstrated that MjTX-II reduced cell proliferation and inhibited fundamental processes for lung cancer cells (A549) growth and metastasis, such as adhesion, migration, invasion, and actin cytoskeleton decrease, without significantly interfering with non-tumorigenic lung cells (BEAS-2B). Furthermore, MjTX-II caused cell cycle alterations, increased reactive oxygen species production, modulated the expression of pro- and antiangiogenic genes, and decreased vascular endothelial growth factor (VEGF) expression in HUVECs. Finally, MjTX-II inhibited ex vivo angiogenesis processes in an aortic ring model. Therefore, we conclude that MjTX-II exhibits antimetastatic and antiangiogenic effects in vitro and ex vivo and represents a molecule that hold promise as a pharmacological model for antitumor therapy.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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Databáze: MEDLINE