Evaluation of a miniature mass spectrometer based point-of-care-test method for direct analysis of amlodipine and benazepril in whole blood.

Autor: Wang S; Key Laboratory of Precision Medicine for Atherosclerotic Diseases of Zhejiang Province, Department of Cardiology, The First Affiliated Hospital of Ningbo University, Ningbo 315000, China., Wu J; Laboratory of Food Nutrition and Clinical Research, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China., Wang Q; Hangzhou Linping Hospital of Traditional Chinese Medicine, Linping, Zhejiang 311106, China., Zhang Y; Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China., Yuan H; First People's Hospital of Linping District, Hangzhou, China., Wang J; Hangzhou Linping Hospital of Traditional Chinese Medicine, Linping, Zhejiang 311106, China., Wu Y; Hangzhou Linping Hospital of Traditional Chinese Medicine, Linping, Zhejiang 311106, China., Xu Y; Central Hospital of Haining, Haining 314408, China., Ji N; Hangzhou Linping Hospital of Traditional Chinese Medicine, Linping, Zhejiang 311106, China., Quan B; Hangzhou Linping Hospital of Traditional Chinese Medicine, Linping, Zhejiang 311106, China. Electronic address: hlhtcm_binquan@163.com., Wang H; Key Laboratory of Drug Monitoring and Control of Zhejiang Province, National Anti-Drug Laboratory Zhejiang Regional Center, Hangzhou, China. Electronic address: wanghaixing007@hotmail.com., Shen Q; Panvascular Diseases Research Center, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou 324000, China; Laboratory of Food Nutrition and Clinical Research, Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China. Electronic address: leonqshen@163.com.
Jazyk: angličtina
Zdroj: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2024 Aug 01; Vol. 245, pp. 116194. Date of Electronic Publication: 2024 May 03.
DOI: 10.1016/j.jpba.2024.116194
Abstrakt: A miniature mass spectrometer (mMS) based point-of-care testing (POCT) method was evaluated for on-site detecting the hypertension drugs, amlodipine and benazepril. The instrument parameters, including voltage, ISO1, ISO2, and CID, were optimized, under which the target compounds could be well detected in MS 2 . When these two drugs were injected simultaneously, the mutual ionization inhibition and mutual reduction between amlodipine and benazepril were evaluated. This phenomenon was severe on the precursor ions but had a small impact on the product ions, thus making this POCT method suitable for analysis using product ions. Finally, the method was validated and applied. The blood samples from patients were tested one hour after oral administration of the drugs (20 mg), and the benazepril was quantitatively analyzed using a standard curve, with detected concentrations ranging from 190.6 to 210 μg L -1 and a relative standard deviation (RSD) of 8.6 %. In summary, amlodipine has low sensitivity and can only be detected at higher concentrations, while benazepril has high sensitivity, good linearity, and even meets semi-quantitative requirements. The research results of this study are of great clinical significance for monitoring blood drug concentrations during hypertension medication, predicting drug efficacy, and customizing individualized medication plans.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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