Insights into the radioprotective efficacy of Pterocarpus santalinus L. aqueous extract.

Autor: Ghali ENHK; Department of Biochemistry, Sri Venkateswara University, Tirupati 517502, India; Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400085, India; Medicine and Oncology ISU, South Texas Center of Excellence in Cancer Research, School of Medicine, University of Texas Rio Grande Valley, McAllen 78504, TX, USA., Sandopu SK; Department of Biochemistry, Sri Venkateswara University, Tirupati 517502, India., Maurya DK; Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400085, India. Electronic address: dkmaurya@barc.gov.in., Meriga B; Department of Biochemistry, Sri Venkateswara University, Tirupati 517502, India. Electronic address: balaji.meriga@gmail.com.
Jazyk: angličtina
Zdroj: Fitoterapia [Fitoterapia] 2024 Jul; Vol. 176, pp. 105986. Date of Electronic Publication: 2024 May 03.
DOI: 10.1016/j.fitote.2024.105986
Abstrakt: In the present study, we have attempted a comprehensive assessment of the possible radioprotective efficacy of Pterocarpus santalinus aqueous extract (PSAE). All the studied models were gamma-irradiated with prior treatment with PSAE. First, the content of total phenols (4.061 μg/mg gallic acid equivalents), flavonoids (6.616 μg/mg quercetin equivalents), and tannins (0.008 mg/L of PSAE) were determined spectrophotometrically. Second, UHPLC-HRMS analysis was performed to identify the possible radioprotectors. Of those, santalins A & B are known for their usage as natural color in foods and alcoholic beverages identified in PSAE. Treatment was well tolerated with no side effects from PSAE. Later, it was shown that radiation-induced lethality significantly amended in PSAE-treated spleen lymphocytes as evidenced by reduced elevated levels of ROS and lipid peroxidation, restored total thiols and GSH: GSSG, inhibited DNA DSBs and cell death. Furthermore, an immunomodulation study was carried out because radiation exposure induces an inflammatory response. Our study shows that PSAE suppressed concanavalin A-induced T-cell proliferation as evidenced by CFSE dye dilution and CD69 antibody staining methods. Taken together, the current study explored the protective efficacy of PSAE from gamma radiation-inflicted injuries and hence we recommend PSAE as a potent radioprotective formulation.
Competing Interests: Declaration of competing interest The authors declare that they have no known relevant financial or non-financial interests to disclose.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: