Role of endogenous soil microorganisms in controlling antimicrobial resistance after the exposure to treated wastewater.

Autor: Della-Negra O; INRAE, University of Montpellier, LBE, Av. des Étangs, 11100 Narbonne, France; UMR HydroSciences Montpellier, University of Montpellier, IRD, CNRS, 15 Av. Charles Flahault, 34093 Montpellier cedex 5, France. Electronic address: oriane.della-negra@inrae.fr., Camotti Bastos M; INRAE, University of Montpellier, LBE, Av. des Étangs, 11100 Narbonne, France; UMR HydroSciences Montpellier, University of Montpellier, IRD, CNRS, 15 Av. Charles Flahault, 34093 Montpellier cedex 5, France., Bru-Adan V; INRAE, University of Montpellier, LBE, Av. des Étangs, 11100 Narbonne, France., Santa-Catalina G; INRAE, University of Montpellier, LBE, Av. des Étangs, 11100 Narbonne, France., Ait-Mouheb N; INRAE, University of Montpellier, UMR GEAU, 361 rue Jean-François Breton, 34196 Montpellier, France., Chiron S; UMR HydroSciences Montpellier, University of Montpellier, IRD, CNRS, 15 Av. Charles Flahault, 34093 Montpellier cedex 5, France., Heran M; IEM, University of Montpellier, Montpellier, France., Wéry N; INRAE, University of Montpellier, LBE, Av. des Étangs, 11100 Narbonne, France., Patureau D; INRAE, University of Montpellier, LBE, Av. des Étangs, 11100 Narbonne, France.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Jun 25; Vol. 931, pp. 172977. Date of Electronic Publication: 2024 May 03.
DOI: 10.1016/j.scitotenv.2024.172977
Abstrakt: The reuse of treated wastewater (TWW) for irrigation appears to be a relevant solution to the challenges of growing water demand and scarcity. However, TWW contains not only micro-pollutants including pharmaceutical residues but also antibiotic resistant bacteria. The reuse of TWW could contribute to the dissemination of antimicrobial resistance in the environment. The purpose of this study was to assess if exogenous bacteria from irrigation waters (TWW or tap water-TP) affect endogenous soil microbial communities (from 2 soils with distinct irrigation history) and key antibiotic resistance gene sul1 and mobile genetic elements intl1 and IS613. Experiments were conducted in microcosms, irrigated in one-shot, and monitored for three months. Results showed that TP or TWW exposure induced a dynamic response of soil microbial communities but with no significant increase of resistance and mobile gene abundances. However, no significant differences were observed between the two water types in the current experimental design. Despite this, the 16S rDNA analysis of the two soils irrigated for two years either with tap water or TWW resulted in soil microbial community differentiation and the identification of biomarkers from Xanthomonadaceae and Planctomycetes families for soils irrigated with TWW. Low-diversity soils were more sensitive to the addition of TWW. Indeed, TWW exposure stimulated the growth of bacterial genera known to be pathogenic, correlating with a sharp increase in the copy number of selected resistance genes (up to 3 logs). These low-diversity soils could thus enable the establishment of exogenous bacteria from TWW which was not observed with native soils. In particular, the emergence of Planctomyces, previously suggested as a biomarker of soil irrigated by TWW, was here demonstrated. Finally, this study showed that water input frequency, initial soil microbial diversity and soil history drive changes within soil endogenous communities and the antibiotic resistance gene pool.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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