Elevated neutrophils and reduced NK cells are associated with altered tear molecular signatures and clinical sequelae of chronic ocular Stevens-Johnson syndrome.

Autor: D'Souza S; Department of Cornea and Refractive Surgery, Narayana Nethralaya, Bangalore, India., Nair AP; GROW Research Laboratory, Narayana Netralaya Foundation, Bangalore, India; Manipal Academy of Higher Education, Manipal, India., Ashok N; GROW Research Laboratory, Narayana Netralaya Foundation, Bangalore, India., Kannan R; GROW Research Laboratory, Narayana Netralaya Foundation, Bangalore, India., Dickman MM; University Eye Clinic Maastricht, Maastricht University Medical Center, Maastricht, the Netherlands; MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, the Netherlands., Nuijts RMMA; University Eye Clinic Maastricht, Maastricht University Medical Center, Maastricht, the Netherlands., Shetty R; Department of Cornea and Refractive Surgery, Narayana Nethralaya, Bangalore, India., Sethu S; GROW Research Laboratory, Narayana Netralaya Foundation, Bangalore, India. Electronic address: swaminathansethu@narayananethralaya.com., Ghosh A; GROW Research Laboratory, Narayana Netralaya Foundation, Bangalore, India. Electronic address: arkasubhra@narayananethralaya.com.
Jazyk: angličtina
Zdroj: The ocular surface [Ocul Surf] 2024 May 03; Vol. 34, pp. 9-21. Date of Electronic Publication: 2024 May 03.
DOI: 10.1016/j.jtos.2024.04.003
Abstrakt: Purpose: Stevens-Johnson syndrome (SJS) is characterised as an immuno-inflammatory condition with potentially blinding ocular sequelae. Therefore, we have investigated the ocular surface immune cell profile and correlated it with secreted tear molecular factors and clinical ocular sequelae in SJS patients.
Methods: 21 patients (42 eyes) with chronic ocular SJS and 16 healthy controls (20 eyes) were included in the study. Severity, types of keratopathies and ocular surface (OS) manifestations were determined. OS wash samples from study subjects were used to determine the status of 13 immune cell subsets using flow cytometry. Levels of 42 secreted immuno-inflammatory factors were measured by flow cytometry-based multiplex ELISA in tear samples.
Results: Neutrophils (Total, activated), neutrophils/NK cells ratio, neutrophils/T cells ratio were significantly (p < 0.05) elevated in SJS, while, proportions of T cells and NKT cells were significantly lower in SJS patients. Positive association between neutrophils and chronic ocular surface complication score (COCS) was observed, whereas, a negative association was noted between NK cells and COCS. Tear fluid levels of IL-6, IL-8, IL-18, IFNα/β/γ, TNFα, LIF, IL-8, HGF, sTNFR-I, NGAL, Granzyme, Perforins, MMP9/TIMP1 ratio were significantly higher in SJS. Loss of Limbal niche correlated significantly with immune profile and clinical sequelae. Increased neutrophils, decreased NK cells and specific set of altered secreted immuno-inflammatory mediators including bFGF, and IL-8 were observed in SJS patients with different types of keratopathies compared to those without keratopathy.
Conclusion: Distinct ocular surface immune profile variations were observed to correlate with clinical stages of chronic ocular SJS. Our findings uncover novel mechanisms and potential for targeted therapy in chronic ocular SJS patients.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: