FURIN, IFNL4, and TLR2 gene polymorphisms in relation to COVID-19 severity: a case-control study in Egyptian patients.

Autor: Elgedawy GA; Department of Clinical Biochemistry and Molecular Diagnostics, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia, Egypt., Elabd NS; Faculty of Medicine, Department of Tropical Medicine, Menoufia University, Shebin El-Kom, Menoufia, 32511, Egypt. naglaa.alabd.12@med.menofia.edu.eg., Salem RH; Department of Clinical Microbiology and Immunology, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia, Egypt., Awad SM; Department of Clinical Microbiology and Immunology, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia, Egypt., Amer AA; Faculty of Medicine, Department of Tropical Medicine, Menoufia University, Shebin El-Kom, Menoufia, 32511, Egypt., Torayah MM; Faculty of Medicine, Department of Critical Care Medicine, Menoufia University, Shebin El-Kom, Egypt., El-Koa AA; Faculty of Medicine, Department of Chest Diseases and Tuberculosis, Menoufia University, Shebin El‑Kom, Menoufia, Egypt., Abozeid M; Department of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia, 32511, Egypt. dr.maiabozeid@gmail.com., Montaser BA; Faculty of Medicine, Department of Clinical Pathology, Menoufia University, Shebin El-Kom, Menoufia, Egypt., Aboshabaan HS; Ph.D. of Biochemistry, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia, Egypt., Abdelkreem M; Department of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia, 32511, Egypt., Helal ML; Department of Clinical Biochemistry and Molecular Diagnostics, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia, Egypt.
Jazyk: angličtina
Zdroj: Infection [Infection] 2024 May 04. Date of Electronic Publication: 2024 May 04.
DOI: 10.1007/s15010-024-02266-1
Abstrakt: Background and Aim: A wide range of clinical manifestations and outcomes, including liver injury, have been reported in COVID-19 patients. We investigated the association of three substantial gene polymorphisms (FURIN, IFNL4, and TLR2) with COVID-19 disease susceptibility and severity to help predict prognosis.
Methods: 150 adult COVID-19-assured cases were categorized as follows: 78 patients with a non-severe presentation, 39 patients with severe disease, and 33 critically ill patients. In addition, 74 healthy controls were included. Clinical and laboratory evaluations were carried out, including complete and differential blood counts, D-dimer, lactate dehydrogenase (LDH), C-reactive protein (CRP), procalcitonin, ferritin, interleukin-6 (Il-6), and liver and kidney functions. FURIN (rs6226), IFNL4 (rs12979860), and TLR2 (rs3804099) genotyping allelic discrimination assays were conducted using real-time PCR.
Results: The FURIN, IFNL4, and TLR2 genotypes and their alleles differed significantly between COVID-19 patients and controls, as well as between patients with severe or critical illness and those with a non-severe presentation. According to a multivariable regression analysis, FURIN (C/T + T/T) and TLR2 (T/C + C/C) mutants were associated with COVID-19 susceptibility, with odds ratios of 3.293 and 2.839, respectively. FURIN C/C and IFNL4 T/T mutants were significantly linked to severe and critical illnesses. Multivariate regression analysis showed that FURIN (G/C + C/C) genotypes and IFNL4 T/T homozygosity were independent risk factors associated with increased mortality.
Conclusion: FURIN, IFNL4, and TLR2 gene variants are associated with the risk of COVID-19 occurrence as well as increased severity and poor outcomes in Egyptian patients.
(© 2024. The Author(s).)
Databáze: MEDLINE
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