Electroacupuncture improves apoptosis of nucleus pulposus cells via the IL-22/JAK2-STAT3 signaling pathway in a rat model of cervical intervertebral disk degeneration.

Autor:	Yan S; Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China., Xie LY; Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China., Duan XX; Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China., Tan JX; Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China., Yang S; Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China., Meng L; Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China., Zhong QH; Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China., Lin WD; Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China., Yang JN; Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China., Xiao YY; Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China., Jiang X; Acupuncture Department II, Yueyang Hospital of Traditional Chinese Medicine, Yueyang, China.
Jazyk:	angličtina
Zdroj:	Acupuncture in medicine : journal of the British Medical Acupuncture Society [Acupunct Med] 2024 Jun; Vol. 42 (3), pp. 146-154. Date of Electronic Publication: 2024 May 03.
DOI:	10.1177/09645284241248465
Abstrakt:	Background: Cervical spondylosis (CS) is a prevalent disorder that can have a major negative impact on quality of life. Traditional conservative treatment has limited efficacy, and electroacupuncture (EA) is a novel treatment option. We investigated the application and molecular mechanism of EA treatment in a rat model of cervical intervertebral disk degeneration (CIDD). Methods: The CIDD rat model was established, following which rats in the electroacupuncture (EA) group received EA. For overexpression of IL-22 or inhibition of JAK2-STAT3 signaling, the rats were injected intraperitoneally with recombinant IL-22 protein (p-IL-22) or the JAK2-STAT3 (Janus kinase 2-signal transducer and activator of transcription protein 3) inhibitor AG490 after model establishment. Rat nucleus pulposus (NP) cells were isolated and cultured. Cell counting kit-8 and flow cytometry were used to analyze the viability and apoptosis of the NP cells. Expression of IL-22, JAK2 and STAT3 was determined using RT-qPCR. Expression of IL-22/JAK2-STAT3 pathway and apoptosis related proteins was detected by Western blotting (WB). Results: EA protected the NP tissues of CIDD rats by regulating the IL-22/JAK2-STAT3 pathway. Overexpression of IL-22 significantly promoted the expression of tumor necrosis factor (TNF)-α, IL-6, IL-1β, matrix metalloproteinase (MMP)3 and MMP13 compared with the EA group. WB demonstrated that the expression of IL-22, p-JAK2, p-STAT3, caspase-3 and Bax in NP cells of the EA group was significantly reduced and Bcl-2 elevated compared with the model group. EA regulated cytokines and MMP through activation of IL-22/JAK2-STAT3 signaling in CIDD rat NP cells. Conclusion: We demonstrated that EA affected apoptosis by regulating the IL-22/JAK2-STAT3 pathway in NP cells and reducing inflammatory factors in the CIDD rat model. The results extend our knowledge of the mechanisms of action underlying the effects of EA as a potential treatment approach for CS in clinical practice. Competing Interests: Declaration of conflicting interestsThe authors declared no potential conflicts of interest with respect to the research, authorship and/or publication of this article.
Databáze:	MEDLINE
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