Simultaneous detection of bovine viral diarrhea virus (BVDV) and bovine herpesvirus 1 (BoHV-1) using recombinase polymerase amplification.

Autor: Jiang L; School of Life Sciences, Ningxia University, Yinchuan, China.; Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan, China., Zhang G; School of Life Sciences, Ningxia University, Yinchuan, China.; Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan, China., Wang P; School of Life Sciences, Ningxia University, Yinchuan, China.; Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan, China., Niu X; School of Life Sciences, Ningxia University, Yinchuan, China.; Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan, China., Liu Q; School of Life Sciences, Ningxia University, Yinchuan, China.; Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan, China., Zhang S; School of Life Sciences, Ningxia University, Yinchuan, China.; Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan, China., Gao W; School of Life Sciences, Ningxia University, Yinchuan, China.; Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan, China., Li Y; School of Life Sciences, Ningxia University, Yinchuan, China. liyong7732@nxu.edu.cn.; Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan, China. liyong7732@nxu.edu.cn.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 May 03; Vol. 14 (1), pp. 10169. Date of Electronic Publication: 2024 May 03.
DOI: 10.1038/s41598-024-56869-7
Abstrakt: Bovine viral diarrhea virus (BVDV) is considered to be the most common agent of severe diarrhea in cattle worldwide, causing fever, diarrhea, ulcers, and abortion. Bovine herpesvirus 1 (BoHV-1) is also a major bovine respiratory disease agent that spreads worldwide and causes extensive damage to the livestock industry. Recombinase polymerase amplification (RPA) is a novel nucleic acid amplification method with the advantages of high efficiency, rapidity and sensitivity, which has been widely used in the diagnosis of infectious diseases. A dual RPA assay was developed for the simultaneous detection of BVDV and BoHV-1. The assay was completed at a constant temperature of 37 °C for 30 min. It was highly sensitive and had no cross-reactivity with other common bovine viruses. The detection rate of BVDV RPA in clinical samples (36.67%) was higher than that of PCR (33.33%), the detection rate of BoHV-1 RPA and PCR were equal. Therefore, the established dual RPA assay for BVDV and BoHV-1 could be a potential candidate for use as an immediate diagnostic.
(© 2024. The Author(s).)
Databáze: MEDLINE
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