Development of transgenic Paulownia trees expressing antimicrobial thionin genes for enhanced resistance to fungal infections using chitosan nanoparticles.

Autor: Bouqellah NA; Taibah University, Science College, Biology Department, 42317- 8599, Al Madinah Al Munawwarah, Saudi Arabia. Electronic address: Nbouqellah@taibahu.edu.sa., Hussein ET; Botany and Microbiology Department, Faculty of Science, Helwan University, Egypt., Abdel Razik AB; Genetic Department, Faculty of Agriculture, Ain-Shams University, Egypt., Ahmed MF; Dry and Saline Farming Technology, Arid Land Agricultural Graduate Studies and Research Institute, Ain Shams University, 11566, Egypt., Faraag AHI; Botany and Microbiology Department, Faculty of Science, Helwan University, Egypt; School of Biotechnology, Badr University in Cairo, Badr City, Cairo, 11829, Egypt. Electronic address: professor_ahmed85@science.helwan.edu.eg.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2024 Jun; Vol. 191, pp. 106659. Date of Electronic Publication: 2024 May 01.
DOI: 10.1016/j.micpath.2024.106659
Abstrakt: There is an increasing focus on genetically altering Paulownia trees to enhance their resistance against fungal infections, given their rapid growth and quality wood production. The aim of this research was to establish a technique for incorporating two antimicrobial thionin genes, namely thionin-60 (thio-60) and thionin-63 (thio-63), into Paulownia tomentosa and Paulownia hybrid 9501 through the utilization of chitosan nanoparticles. The outcomes revealed the successful gene transfer into Paulownia trees utilizing chitosan nanoparticles. The effectiveness of thionin proteins against plant pathogens Fusarium and Aspergillus was examined, with a specific focus on Fusarium equiseti due to limited available data. In non-transgenic Paulownia species, the leaf weight inhibition percentage varied from 25 to 36 %, whereas in transgenic species, it ranged from 22 to 7 %. In general, Paulownia species expressing thio-60 displayed increased resistance to F. equiseti, while those expressing thio-63 exhibited heightened resistance to A. niger infection. The thionin proteins displayed a strong affinity for the phospholipid bilayer of the fungal cell membrane, demonstrating their capability to disrupt its structure. The transgenic plants created through this technique showed increased resistance to fungal infections. Thionin-60 demonstrated superior antifungal properties in comparison to thio-63, being more effective at disturbing the fungal cell membrane. These findings indicate that thio-60 holds potential as a novel antifungal agent and presents a promising approach for enhancing the antimicrobial traits of genetically modified Paulownia trees.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Nahla Alsayd Bouqellah reports administrative support was provided by Taibah University, Science College, Biology Department, 42317-8599, Al Madinah Al Munawwarah, Saudi Arabia. Nahla Alsayd Bouqellah reports a relationship with Taibah University, Science College, Biology Department, 42317-8599, Al Madinah Al Munawwarah, Saudi Arabia that includes: employment. No conflict of interest exists. The content and composition of this work are my responsibility.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE