Effect of ruthenium(II) complexes on MDA-MB-231 cells and lifespan/tumor growth in gld-1mutant, Daf-16 TF and stress productive genes: A perspective study.

Autor: Nandhini S; Department of Chemistry, Bharathiar University, Coimbatore 641 046, India., Thiruppathi G; Department of Zoology, Bharathiar University, Coimbatore 641 046, India., Ranjani M; Department of Chemistry, Bharathiar University, Coimbatore 641 046, India., Puschmann H; Department of Chemistry, Durham University, Durham DH1 3LE, UK., Ravi M; Department of Biochemistry, University of Madras, Guindy Campus, Chennai 600025, India., Sundararaj P; Department of Zoology, Bharathiar University, Coimbatore 641 046, India., Prabhakaran R; Department of Chemistry, Bharathiar University, Coimbatore 641 046, India. Electronic address: rpnchemist@gmail.com.
Jazyk: angličtina
Zdroj: Journal of inorganic biochemistry [J Inorg Biochem] 2024 Aug; Vol. 257, pp. 112580. Date of Electronic Publication: 2024 Apr 28.
DOI: 10.1016/j.jinorgbio.2024.112580
Abstrakt: Pincer type coumarin based N-substituted semicarbazone ligands HL 1-4 and their corresponding ruthenium(II) complexes (1-4) were synthesized, analyzed and confirmed by various spectro analytical techniques. The molecular structure of the ligand HL 3 and complex 3 was confirmed by single crystal X-ray diffraction analysis. The stoichiometry of complexes 1, 2 and 4 was confirmed by high resolution mass spectroscopy (HRMS). The binding affinity of the compounds with CT-DNA (Calf Thymus DNA) and BSA (Bovine Serum Albumin) was established by absorption and emission titration methods. The results of In vitro cytotoxicity showed the significant cytotoxic potential of the complexes against MDA-MB-231 cells (TNBC- Triple-negative breast cancer). Among the complexes, 1 and 4 have shown appreciable results. Further, antimigratory activity against the MDA-MB-231 cells was studied for the complexes 1 and 4. The percentage cell cycle arrest, apoptosis and necrosis were explored by flow cytometry. The in vivo anti-tumor activity of the complexes 1 and 4 using C. elegans as model organism was established by using the tumoral C. elegans strain JK1466 (gld-1(q485)), which bears a mutation in the gld-1 tumor suppressor gene. We have determined the effect of our complexes on tumor gonad reduction and found to be non toxic to the JK1466 worms and they have prolonged their mean lifespan with potential antioxidant ability by overcoming stress responses. Overall, our study reported herein demonstrated that the complexes 1 and 4 could be established as potential metallo-drugs substantiating further exploration.
Competing Interests: Declaration of competing interest We authors declare that we are having any conflicts of interest in publishing this current manuscript in Journal of Inorganic Biochemistry as full research article.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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