Membrane-Bound Ferric Hemoglobin in Nucleated Erythrocytes of the Black Scorpionfish Scorpaena porcus, Linnaeus 1758.

Autor: Soldatov AA; Kovalevsky Institute of Biology of the South Seas, Russian Academy of Sciences, Sevastopol, Russia. alekssoldatov@yandex.ru.; Sevastopol State University, Sevastopol, Russia. alekssoldatov@yandex.ru., Shalagina NE; Kovalevsky Institute of Biology of the South Seas, Russian Academy of Sciences, Sevastopol, Russia., Rychkova VN; Kovalevsky Institute of Biology of the South Seas, Russian Academy of Sciences, Sevastopol, Russia., Kukhareva TA; Kovalevsky Institute of Biology of the South Seas, Russian Academy of Sciences, Sevastopol, Russia.
Jazyk: angličtina
Zdroj: Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections [Dokl Biol Sci] 2024 Jun; Vol. 516 (1), pp. 50-54. Date of Electronic Publication: 2024 May 03.
DOI: 10.1134/S0012496624700984
Abstrakt: The content of membrane-bound methemoglobin (MtHb) in nucleated erythrocytes was studied in the black scorpionfish Scorpaena porcus (Linnaeus, 1758) in vitro. Spectral characteristics were determined for a whole hemolysate, a hemolysate obtained by stroma precipitation (a clarified hemolysate), and a resuspended stroma. The MtHb proportion in the erythrocyte stroma was found to exceed 80% (6.20 ± 0.59 µM). Clarified hemolysates were nearly free of MtHb (0.5 ± 0.2 µM). Membrane-bound ferric hemoglobin did not affect the erythrocyte resistance to osmotic shock. The osmotic fragility range was determined using a LaSca-TM laser microparticle analyzer (BioMedSystems, Russia) to be 102-136 mOsm/kg, much the same as in other bony fish species. A nitrite load (10 mg/L) significantly increased the MtHb content in the blood. However, the membrane-bound ferric hemoglobin content did not change significantly, amounting to 6.34 ± 1.09 µM (approximately 95%). The finding suggested a functional importance for MtHb present in the plasma membrane of nucleated erythrocytes. Membrane-bound MtHb was assumed to neutralize the external oxidative load and the toxic effect of hydrogen sulfide in bottom water layers, where the species lives.
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Databáze: MEDLINE
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