Regulation of B-cell function and expression of CD11c, T-bet, and FcRL5 in response to different activation signals.

Autor: Kleberg L; Division of Infectious Diseases, Department of Medicine, Solna, Karolinska Institutet, Stockholm, Sweden.; Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.; Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden., Courey-Ghaouzi AD; Division of Infectious Diseases, Department of Medicine, Solna, Karolinska Institutet, Stockholm, Sweden.; Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.; Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden., Lautenbach MJ; Division of Infectious Diseases, Department of Medicine, Solna, Karolinska Institutet, Stockholm, Sweden.; Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.; Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden., Färnert A; Division of Infectious Diseases, Department of Medicine, Solna, Karolinska Institutet, Stockholm, Sweden.; Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.; Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden., Sundling C; Division of Infectious Diseases, Department of Medicine, Solna, Karolinska Institutet, Stockholm, Sweden.; Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.; Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden.
Jazyk: angličtina
Zdroj: European journal of immunology [Eur J Immunol] 2024 Aug; Vol. 54 (8), pp. e2350736. Date of Electronic Publication: 2024 May 03.
DOI: 10.1002/eji.202350736
Abstrakt: CD11c, FcRL5, or T-bet are commonly expressed by B cells expanding during inflammation, where they can make up >30% of mature B cells. However, the association between the proteins and differentiation and function in the host response remains largely unclear. We have assessed the co-expression of CD11c, T-bet, and FcRL5 in an in vitro B-cell culture system to determine how stimulation via the BCR, toll-like receptor 9 (TLR9), and different cytokines influence CD11c, T-bet, and FcRL5 expression. We observed different expression dynamics for all markers, but a largely overlapping regulation of CD11c and FcRL5 in response to BCR and TLR9 activation, while T-bet was strongly dependent on IFN-γ signaling. Investigating plasma cell differentiation and APC functions, there was no association between marker expression and antibody secretion or T-cell help. Rather the functions were associated with TLR9-signalling and B-cell-derived IL-6 production, respectively. These results suggest that the expression of CD11c, FcRL5, and T-bet and plasma cell differentiation and improved APC functions occur in parallel and are regulated by similar activation signals, but they are not interdependent.
(© 2024 The Authors. European Journal of Immunology published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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