Protecting the piglet gut microbiota against ETEC-mediated post-weaning diarrhoea using specific binding proteins.

Autor: Jenkins TP; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark., Ács N; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark., Arendrup EW; Bactolife A/S, Rønnegade 8, Copenhagen, Denmark., Swift A; Veterinary Sciences, University of Bristol, Bristol, UK., Duzs Á; Bactolife A/S, Rønnegade 8, Copenhagen, Denmark., Chatzigiannidou I; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark., Pichler M; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark., Kittilä T; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark., Peachey L; Veterinary Sciences, University of Bristol, Bristol, UK., Gram L; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark., Canibe N; Department of Animal Science, Aarhus University, Tjele, Denmark., Laustsen AH; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark. ahola@bio.dtu.dk.; Bactolife A/S, Rønnegade 8, Copenhagen, Denmark. ahola@bio.dtu.dk., Brix S; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark. sbp@bio.dtu.dk., Thrane SW; Bactolife A/S, Rønnegade 8, Copenhagen, Denmark. swt@bactolife.com.
Jazyk: angličtina
Zdroj: NPJ biofilms and microbiomes [NPJ Biofilms Microbiomes] 2024 May 02; Vol. 10 (1), pp. 42. Date of Electronic Publication: 2024 May 02.
DOI: 10.1038/s41522-024-00514-8
Abstrakt: Post-weaning diarrhoea (PWD) in piglets presents a widespread problem in industrial pig production and is often caused by enterotoxigenic E. coli (ETEC) strains. Current solutions, such as antibiotics and medicinal zinc oxide, are unsustainable and are increasingly being prohibited, resulting in a dire need for novel solutions. Thus, in this study, we propose and evaluate a protein-based feed additive, comprising two bivalent heavy chain variable domain (V H H) constructs (V H H-(GGGGS) 3 -V H H, BL1.2 and BL2.2) as an alternative solution to manage PWD. We demonstrate in vitro that these constructs bind to ETEC toxins and fimbriae, whilst they do no affect bacterial growth rate. Furthermore, in a pig study, we show that oral administration of these constructs after ETEC challenge reduced ETEC proliferation when compared to challenged control piglets (1-2 log 10 units difference in gene copies and bacterial count/g faeces across day 2-7) and resulted in week 1 enrichment of three bacterial families (Prevotellaceae (estimate: 1.12 ± 0.25, q = 0.0054), Lactobacillaceae (estimate: 2.86 ± 0.52, q = 0.0012), and Ruminococcaceae (estimate: 0.66 ± 0.18, q = 0.049)) within the gut microbiota that appeared later in challenged control piglets, thus pointing to an earlier transition towards a more mature gut microbiota. These data suggest that such V H H constructs may find utility in industrial pig production as a feed additive for tackling ETEC and reducing the risk of PWD in piglet populations.
(© 2024. The Author(s).)
Databáze: MEDLINE
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