Analyses of long-term fungal degradation of spruce bark reveals varying potential for catabolism of polysaccharides and extractive compounds.

Autor: Ristinmaa AS; Chalmers University of Technology, Department of Life Sciences, Division of Industrial Biotechnology, SE-412 96 Gothenburg, Sweden., Korotkova E; Åbo Akademi University, Laboratory of Natural Materials Technology, FI-20500 Åbo, Finland., Arntzen MØ; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), NO-1433 Ås, Norway., G H Eijsink V; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (NMBU), NO-1433 Ås, Norway., Xu C; Åbo Akademi University, Laboratory of Natural Materials Technology, FI-20500 Åbo, Finland., Sundberg A; Åbo Akademi University, Laboratory of Natural Materials Technology, FI-20500 Åbo, Finland., Hasani M; Department Chemistry and Chemical Engineering, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden; Wallenberg Wood Science Center, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden., Larsbrink J; Chalmers University of Technology, Department of Life Sciences, Division of Industrial Biotechnology, SE-412 96 Gothenburg, Sweden; Wallenberg Wood Science Center, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden. Electronic address: johan.larsbrink@chalmers.se.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2024 Jun; Vol. 402, pp. 130768. Date of Electronic Publication: 2024 Apr 30.
DOI: 10.1016/j.biortech.2024.130768
Abstrakt: The bark represents the outer protective layer of trees. It contains high concentrations of antimicrobial extractives, in addition to regular wood polymers. It represents a huge underutilized side stream in forestry, but biotechnological valorization is hampered by a lack of knowledge on microbial bark degradation. Many fungi are efficient lignocellulose degraders, and here, spruce bark degradation by five species, Dichomitus squalens, Rhodonia placenta, Penicillium crustosum, Trichoderma sp. B1, and Trichoderma reesei, was mapped, by continuously analyzing chemical changes in the bark over six months. The study reveals how fungi from different phyla degrade bark using diverse strategies, regarding both wood polymers and extractives, where toxic resin acids were degraded by Basidiomycetes but unmodified/tolerated by Ascomycetes. Proteome analyses of the white-rot D. squalens revealed several proteins, with both known and unknown functions, that were specifically upregulated during growth on bark. This knowledge can accelerate improved utilization of an abundant renewable resource.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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