Eosinophilic globules (syn. hyaline inclusions) and protein crystalloids in the nasal mucosa of rats treated with synthetic amorphous silica, an unspecific chitinase-like-protein-positive background lesion.

Autor: Weber K; AnaPath Services GmbH, Liestal, Switzerland. Electronic address: kweber@anapath.ch., Warfving N; AnaPath Services GmbH, Liestal, Switzerland., Bruer GG; Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Germany., Krueger N; Evonik Operations GmbH, Smart Materials, Hanau, Germany., Okazaki Y; AnaPath Services GmbH, Liestal, Switzerland., Schoenauer R; AnaPath Services GmbH, Liestal, Switzerland., Schaudien D; Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Germany.
Jazyk: angličtina
Zdroj: Toxicology letters [Toxicol Lett] 2024 Apr 30. Date of Electronic Publication: 2024 Apr 30.
DOI: 10.1016/j.toxlet.2024.04.012
Abstrakt: In a 13-week inhalation toxicity study with three recovery periods (3, 6, and 12 months), Crl: WI rats were allocated to nine groups, each containing 25 animals per sex. Eight groups were treated daily by inhalation with the test items at concentrations of 0.5, 1.0, 2.5, or 5.0 mg/m 3 (SAS 1 groups 2, 3, 4, or 5, respectively; SAS 2 groups 6, 7, 8, or 9, respectively). Controls (group 1) were treated with air only. In nasal cavities, the major lesions consisted of increased eosinophilic globules and chitinase-3-like-protein-positive crystalloids* in the nasal mucosa, mainly in nasal cavity levels 2-4 up to week 26 of recovery without any further injury in olfactory mucosa, mainly in SAS 1-treated animals. Eosinophilic globules in the rodent nasal cavity are common and increase with age; they represent a particular finding of the rodent nasal mucosa. The relevance of chitinase-3-like protein (Ym1 + Ym2) expression in the rodent nasal mucosa is unknown but is normal in control animals. Both findings developed without any indicator for inflammatory processes. The increase of these unspecific background findings is considered an indicator of minor irritative effects. Due to the clear lack of nasal tissue injury or concurrent changes (degeneration, necrosis, inflammatory infiltrate, dysplasia, and/or neoplasia) following repeated inhalation exposure to SAS, it is deemed that the eosinophilic globules (hyaline inclusions) combined with the formation of eosinophilic protein crystalloids in this study represent an adaptive response.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests. Authors reports financial support was provided by SASforREACH, a consortium of the producers of synthetic amorphous silica. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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