Emergence of aztreonam/avibactam and tigecycline-resistant Pseudomonas putida group Co-producing bla IMP-1 , bla AFM-4 and bla OXA-1041 with a novel sequence type ST268 in Southwestern China.

Autor: Jian C; Department of Laboratory Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, China; Sichuan Province Engineering Technology Research Center of Molecular Diagnosis of Clinical Diseases, Luzhou, China; Molecular Diagnosis of Clinical Diseases Key Laboratory of Luzhou, China. Electronic address: jcxlml2018@163.com., Ye C; Department of Laboratory Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, China; Sichuan Province Engineering Technology Research Center of Molecular Diagnosis of Clinical Diseases, Luzhou, China; Molecular Diagnosis of Clinical Diseases Key Laboratory of Luzhou, China. Electronic address: yecaihong1997@swmu.edu.cn., Guo T; Department of Laboratory Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, China; Sichuan Province Engineering Technology Research Center of Molecular Diagnosis of Clinical Diseases, Luzhou, China; Molecular Diagnosis of Clinical Diseases Key Laboratory of Luzhou, China. Electronic address: guotongtong0822@163.com., Hao J; Department of Laboratory Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, China; Sichuan Province Engineering Technology Research Center of Molecular Diagnosis of Clinical Diseases, Luzhou, China; Molecular Diagnosis of Clinical Diseases Key Laboratory of Luzhou, China. Electronic address: haojc1@163.com., Ding Y; Department of Laboratory Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, China; Sichuan Province Engineering Technology Research Center of Molecular Diagnosis of Clinical Diseases, Luzhou, China; Molecular Diagnosis of Clinical Diseases Key Laboratory of Luzhou, China. Electronic address: dingyinhuan@swmu.edu.cn., Xiao X; Department of Laboratory Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, China; Sichuan Province Engineering Technology Research Center of Molecular Diagnosis of Clinical Diseases, Luzhou, China; Molecular Diagnosis of Clinical Diseases Key Laboratory of Luzhou, China. Electronic address: Xiaoxue202100@163.com., Xie W; Department of Laboratory Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, China; Sichuan Province Engineering Technology Research Center of Molecular Diagnosis of Clinical Diseases, Luzhou, China; Molecular Diagnosis of Clinical Diseases Key Laboratory of Luzhou, China. Electronic address: xiewenchao2001@163.com., Zeng Z; Department of Laboratory Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, China; Sichuan Province Engineering Technology Research Center of Molecular Diagnosis of Clinical Diseases, Luzhou, China; Molecular Diagnosis of Clinical Diseases Key Laboratory of Luzhou, China. Electronic address: zengzhangrui@swmu.edu.cn., Liu J; Department of Laboratory Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, China; Sichuan Province Engineering Technology Research Center of Molecular Diagnosis of Clinical Diseases, Luzhou, China; Molecular Diagnosis of Clinical Diseases Key Laboratory of Luzhou, China. Electronic address: liujb7203@swmu.edu.cn.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2024 Jul; Vol. 192, pp. 106668. Date of Electronic Publication: 2024 Apr 30.
DOI: 10.1016/j.micpath.2024.106668
Abstrakt: Objectives: The emergence of carbapenem-resistant Pseudomonas putida (CRPP) has raised public awareness. This study investigated two strains from the Pseudomonas putida group that were resistant to carbapenem, tigecycline, and aztreonam-avibactam (ATM-AVI), with a focus on their microbial and genomic characteristics.
Methods: We assessed the antibiotic resistance profile using broth dilution, disk diffusion, and E-test methods. Efflux pump phenotype testing and real-time quantitative PCR were employed to evaluate efflux pump activity in tigecycline resistance, while polymerase chain reaction was utilized to detect common carbapenem genes. Additionally, whole-genome sequencing was performed to analyze genomic characteristics. The transferability of bla IMP-1 and bla AFM-4 was assessed through a conjugation experiment. Furthermore, growth kinetics and biofilm formation were examined using growth curves and crystal violet staining.
Results: Both strains demonstrated resistance to carbapenem, tigecycline, and ATM-AVI. Notably, NMP can restore sensitivity to tigecycline. Subsequent analysis revealed that they co-produced bla IMP-1 , bla AFM-4 , tmexCD-toprJ, and bla OXA-1041 , belonging to a novel sequence type ST268. Although they were closely related on the phylogenetic tree, they exhibited different levels of virulence. Genetic environment analysis indicated variations compared to prior studies, particularly regarding the bla IMP-1 and bla AFM-4 genes, which showed limited horizontal transferability. Moreover, it was observed that temperature exerted a specific influence on their biological factors.
Conclusion: We initially identified two P. putida ST268 strains co-producing bla IMP-1 , bla AFM-4 , bla OXA-1041 , and tmexCD-toprJ. The resistance to tigecycline and ATM-AVI can be attributed to the presence of multiple drug resistance determinants. These findings underscore the significance of P. putida as a reservoir for novel antibiotic resistance genes. Therefore, it is imperative to develop alternative antibiotic therapies and establish effective monitoring of bacterial resistance.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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