Red-Light-Induced Genetic System for Control of Extracellular Electron Transfer.

Autor: Zhao F; Department of Physics and Astronomy, University of Southern California, Los Angeles, California 90089, United States., Niman CM; Department of Physics and Astronomy, University of Southern California, Los Angeles, California 90089, United States., Ostovar G; Department of Physics and Astronomy, University of Southern California, Los Angeles, California 90089, United States., Chavez MS; Department of Physics and Astronomy, University of Southern California, Los Angeles, California 90089, United States., Atkinson JT; Department of Physics and Astronomy, University of Southern California, Los Angeles, California 90089, United States.; Department of Civil and Environmental Engineering, Princeton University, Princeton, New Jersey 08540, United States.; Omenn-Darling Bioengineering Institute, Princeton University, Princeton, New Jersey 08540, United States., Bonis BM; BioTechnology Institute and Department of Plant and Microbial Biology, University of Minnesota─Twin Cities, St. Paul, Minnesota 55108, United States., Gralnick JA; BioTechnology Institute and Department of Plant and Microbial Biology, University of Minnesota─Twin Cities, St. Paul, Minnesota 55108, United States., El-Naggar MY; Department of Physics and Astronomy, University of Southern California, Los Angeles, California 90089, United States.; Department of Biological Sciences, University of Southern California, Los Angeles, California 90089, United States.; Department of Chemistry, University of Southern California, Los Angeles, California 90089, United States., Boedicker JQ; Department of Physics and Astronomy, University of Southern California, Los Angeles, California 90089, United States.; Department of Biological Sciences, University of Southern California, Los Angeles, California 90089, United States.
Jazyk: angličtina
Zdroj: ACS synthetic biology [ACS Synth Biol] 2024 May 17; Vol. 13 (5), pp. 1467-1476. Date of Electronic Publication: 2024 May 02.
DOI: 10.1021/acssynbio.3c00684
Abstrakt: Optogenetics is a powerful tool for spatiotemporal control of gene expression. Several light-inducible gene regulators have been developed to function in bacteria, and these regulatory circuits have been ported to new host strains. Here, we developed and adapted a red-light-inducible transcription factor for Shewanella oneidensis . This regulatory circuit is based on the iLight optogenetic system, which controls gene expression using red light. A thermodynamic model and promoter engineering were used to adapt this system to achieve differential gene expression in light and dark conditions within a S. oneidensis host strain. We further improved the iLight optogenetic system by adding a repressor to invert the genetic circuit and activate gene expression under red light illumination. The inverted iLight genetic circuit was used to control extracellular electron transfer within S. oneidensis . The ability to use both red- and blue-light-induced optogenetic circuits simultaneously was also demonstrated. Our work expands the synthetic biology capabilities in S. oneidensis , which could facilitate future advances in applications with electrogenic bacteria.
Databáze: MEDLINE
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