Rapid and specific detection of thiabendazole: enzymatic digestion-enabled fluorescent aptasensor.

Autor: Berkal MA; Universite de Pau Et Des Pays de L'Adour, E2S UPPA, CNRS, IPREM, Pau, France., Toulme JJ; Novaptech, 2 Avenue Favard, 33170, Gradignan, France., Nardin C; Universite de Pau Et Des Pays de L'Adour, E2S UPPA, CNRS, IPREM, Pau, France. corinne.nardin@univ-pau.fr.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2024 Jun; Vol. 416 (14), pp. 3295-3303. Date of Electronic Publication: 2024 May 02.
DOI: 10.1007/s00216-024-05309-6
Abstrakt: Thiabendazole, a widely used broad-spectrum fungicide in agriculture, poses risks to human health. To monitor its presence in water, we propose a fluorescent aptasensor utilizing Escherichia coli exonuclease I (Exo I). The findings demonstrate a linear correlation between thiabendazole concentrations and digestion percentage, with a detection limit (LOD) exceeding 1 µM and a determination coefficient (R 2 ) of 0.959. This aptamer-based fluorescence spectroscopy detection system holds promise for a rapid, specific, and sensitive analysis of thiabendazole in environmental waters and food matrices.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: