| Autor: |
Singh S; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Al-Quwayiyah, Riyadh, Saudi Arabia., Arya H; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Al-Quwayiyah, Riyadh, Saudi Arabia., Sahu W; School of Biotechnology, Kalinga Institute of Industrial Technology (Deemed University), Bhubaneswar, India., Reddy KS; School of Biotechnology, Kalinga Institute of Industrial Technology (Deemed University), Bhubaneswar, India., Nimesh S; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Al-Quwayiyah, Riyadh, Saudi Arabia., Alotaibi BS; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Riyadh, Saudi Arabia., Hakami MA; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Riyadh, Saudi Arabia., Almasoudi HH; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Najran University, Najran, Saudi Arabia., Hessien KBG; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Riyadh, Saudi Arabia., Hasan MR; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Riyadh, Saudi Arabia., Rashid S; Department of Pharmacology and Toxicology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia., Kumar Bhatt T; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Al-Quwayiyah, Riyadh, Saudi Arabia. |
| Abstrakt: |
Malaria is a mosquito-borne infectious disease that is caused by the Plasmodium parasite. Most of the available medication are losing their efficacy. Therefore, it is crucial to create fresh leads to combat malaria. Green silver nanoparticles (AgNPs) have recently attracted a lot of attention in biomedical research. As a result, green mediated AgNPs from leaves of Terminalia bellirica , a medicinal plant with purported antimalarial effects, were used in this investigation. Initially, cysteine-rich proteins from Plasmodium species were studied in silico as potential therapeutic targets. With docking scores between -9.93 and -11.25 kcal/mol, four leaf constituents of Terminalia bellirica were identified. The green mediated silver nanoparticles were afterward produced using leaf extract and were further examined using UV-vis spectrophotometer, DLS, Zeta potential, FTIR, XRD, and FESEM. The size of synthesized TBL-AgNPs was validated by the FESEM results; the average size of TBL-AgNPs was around 44.05 nm. The zeta potential study also supported green mediated AgNPs stability. Additionally, Plasmodium falciparum (3D7) cultures were used to assess the antimalarial efficacy, and green mediated AgNPs could effectively inhibit the parasitized red blood cells (pRBCs). In conclusion, this novel class of AgNPs may be used as a potential therapeutic replacement for the treatment of malaria. |
