| Autor: |
Jiang X; College of Environmental Science and Engineering, China West Normal University, Nanchong 637009, Sichuan Province, PR China., Tang Y; Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Science, China West Normal University, Nanchong 637009, Sichuan Province, PR China., Yang Y; Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Science, China West Normal University, Nanchong 637009, Sichuan Province, PR China., Tang J; Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Science, China West Normal University, Nanchong 637009, Sichuan Province, PR China., Hou M; Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Science, China West Normal University, Nanchong 637009, Sichuan Province, PR China., Liu R; Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Science, China West Normal University, Nanchong 637009, Sichuan Province, PR China., Dong Q; Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Science, China West Normal University, Nanchong 637009, Sichuan Province, PR China., Ma Z; Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Science, China West Normal University, Nanchong 637009, Sichuan Province, PR China. |
| Abstrakt: |
A novel actinobacterium, designated strain CWNU-1 T , was isolated from the rhizospheric soil of Fritillaria cirrhosa D. Don and examined using a polyphasic taxonomic approach. The organism developed pale blue aerial mycelia that was simply branched and terminated in open or closed coils of three or more volutions on International Streptomyces Project 3 agar. Spores were ellipsoidal to cylindrical with wrinkled surfaces. The strain showed high 16S rRNA gene sequence similarity to Streptomyces kurssanovii NBRC 13192 T (98.8 %), Streptomyces xantholiticus NBRC 13354 T (98.7 %) and Streptomyces peucetius JCM 9920 T (98.6 %). The phylogenetic result based on 16S rRNA gene and genome sequences clearly demonstrated that strain CWNU-1 T formed an independent phylogenetic lineage. On the basis of orthologous average nucleotide identity, CWNU-1 T was most closely related to Streptomyces inusitatus NBRC 13601 T with 79.3 % identity. The results of the digital DNA-DNA hybridization analysis also indicated low levels of relatedness with other species, as the highest value was observed with S. inusitatus NBRC 13601 T (25.3 %). With reference to phenotypic characteristics, phylogenetic data, orthologous average nucleotide identity and digital DNA-DNA hybridization results, strain CWNU-1 T was readily distinguished from its most closely related strains and classified as representing a novel species, for which the name Streptomyces albipurpureus sp. nov. is proposed. The type strain is CWNU-1 T (=CGMCC 4.7758 T =MCCC 1K07402 T =JCM 35391 T ). |
