A facile and scalable method to synthesize PEGylated PDMAEMA for gene delivery.

Autor: Dou J; Department of Chemistry and Environmental Science, New Jersey Institute of Technology, Newark, New Jersey, USA., Yu S; Department of Chemistry and Environmental Science, New Jersey Institute of Technology, Newark, New Jersey, USA., Zhang Y; Department of Chemistry and Environmental Science, New Jersey Institute of Technology, Newark, New Jersey, USA.
Jazyk: angličtina
Zdroj: Biopolymers [Biopolymers] 2024 Jul; Vol. 115 (4), pp. e23584. Date of Electronic Publication: 2024 May 02.
DOI: 10.1002/bip.23584
Abstrakt: In recent years, cationic polymer vectors have been viewed as a promising method for delivering nucleic acids. With the advancement of synthetic polymer chemistry, we can control chemical structures and properties to enhance the efficacy of gene delivery. Herein, a facile, cost-effective, and scalable method was developed to synthesize PEGylated PDMAEMA polymers (PEO-PDMAEMA-PEO), where PEGylation could enable prolonged polyplexes circulation time in the blood stream. Two polymers of different molecular weights were synthesized, and polymer/eGFP polyplexes were prepared and characterized. The correlation between polymers' molecular weight and physicochemical properties (size and zeta potential) of polyplexes was investigated. Lipofectamine 2000, a commercial non-viral transfection reagent, was used as a standard control. PEO-PDMAEMA-PEO with higher molecular weight exhibited slightly better transfection efficiency than Lipofectamine 2000, and the cytotoxicity study proved that it could function as a safe gene vector. We believe that PEO-PDMAEMA-PEO could serve as a model to investigate more potential in the gene delivery area.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
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