Mechanics of human embryo compaction.

Autor: Firmin J; Institut Curie, Université PSL, CNRS UMR3215, INSERM U934, Paris, France.; Université de Paris, Paris, France.; Service de Biologie de la Reproduction - CECOS, Paris Centre Hospital, APHP centre, FHU Prema, Paris, France., Ecker N; Center for Interdisciplinary Research in Biology, Collège de France, CNRS, INSERM, Université PSL, FHU Prema, Paris, France., Rivet Danon D; Service de Biologie de la Reproduction - CECOS, Paris Centre Hospital, APHP centre, FHU Prema, Paris, France., Özgüç Ö; Institut Curie, Université PSL, CNRS UMR3215, INSERM U934, Paris, France., Barraud Lange V; Service de Biologie de la Reproduction - CECOS, Paris Centre Hospital, APHP centre, FHU Prema, Paris, France.; Institut Cochin, Université de Paris, CNRS UMR1016, Paris, France., Turlier H; Center for Interdisciplinary Research in Biology, Collège de France, CNRS, INSERM, Université PSL, FHU Prema, Paris, France., Patrat C; Service de Biologie de la Reproduction - CECOS, Paris Centre Hospital, APHP centre, FHU Prema, Paris, France.; Institut Cochin, Université de Paris, CNRS UMR1016, Paris, France., Maître JL; Institut Curie, Université PSL, CNRS UMR3215, INSERM U934, Paris, France. jean-leon.maitre@curie.fr.
Jazyk: angličtina
Zdroj: Nature [Nature] 2024 May; Vol. 629 (8012), pp. 646-651. Date of Electronic Publication: 2024 May 01.
DOI: 10.1038/s41586-024-07351-x
Abstrakt: The shaping of human embryos begins with compaction, during which cells come into close contact 1,2 . Assisted reproductive technology studies indicate that human embryos fail compaction primarily because of defective adhesion 3,4 . On the basis of our current understanding of animal morphogenesis 5,6 , other morphogenetic engines, such as cell contractility, could be involved in shaping human embryos. However, the molecular, cellular and physical mechanisms driving human embryo morphogenesis remain uncharacterized. Using micropipette aspiration on human embryos donated to research, we have mapped cell surface tensions during compaction. This shows a fourfold increase of tension at the cell-medium interface whereas cell-cell contacts keep a steady tension. Therefore, increased tension at the cell-medium interface drives human embryo compaction, which is qualitatively similar to compaction in mouse embryos 7 . Further comparison between human and mouse shows qualitatively similar but quantitively different mechanical strategies, with human embryos being mechanically least efficient. Inhibition of cell contractility and cell-cell adhesion in human embryos shows that, whereas both cellular processes are required for compaction, only contractility controls the surface tensions responsible for compaction. Cell contractility and cell-cell adhesion exhibit distinct mechanical signatures when faulty. Analysing the mechanical signature of naturally failing embryos, we find evidence that non-compacting or partially compacting embryos containing excluded cells have defective contractility. Together, our study shows that an evolutionarily conserved increase in cell contractility is required to generate the forces driving the first morphogenetic movement shaping the human body.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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