Unraveling the phylogenetics of genetically closely related species, Haemaphysalis japonica and Haemaphysalis megaspinosa, using entire tick mitogenomes and microbiomes.

Autor: Moustafa MAM; Department of Entomology, Rutgers School of Environmental and Biological Sciences, Rutgers the State University of New Jersey, New Brunswick, NJ, 08901, USA.; Laboratory of Parasitology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, 060-0818, Japan.; Department of Animal Medicine, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt., Mohamed WMA; Laboratory of Parasitology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, 060-0818, Japan.; Department of Biochemistry and Microbiology, Rutgers School of Environmental and Biological Sciences, Rutgers the State University of New Jersey, New Brunswick, NJ, 08901, USA., Chatanga E; Laboratory of Parasitology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, 060-0818, Japan.; Department of Veterinary Pathobiology, Lilongwe University of Agriculture and Natural Resources, P.O. Box 219, Lilongwe, Malawi., Naguib D; Laboratory of Parasitology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, 060-0818, Japan.; Department of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt., Matsuno K; One Health Research Center, Hokkaido University, Sapporo, Japan.; International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Japan.; Division of Risk Analysis and Management, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Japan.; Institute for Vaccine Research and Development, HU-IVReD, Hokkaido University, Sapporo, Japan., Gofton AW; CSIRO, Health and Biosecurity, Canberra, ACT, Australia., Barker SC; Department of Parasitology, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, 4072, Australia., Nonaka N; Laboratory of Parasitology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, 060-0818, Japan., Nakao R; Laboratory of Parasitology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, 060-0818, Japan. ryo.nakao@vetmed.hokudai.ac.jp.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Apr 30; Vol. 14 (1), pp. 9961. Date of Electronic Publication: 2024 Apr 30.
DOI: 10.1038/s41598-024-60163-x
Abstrakt: Ticks have a profound impact on public health. Haemaphysalis is one of the most widespread genera in Asia, including Japan. The taxonomy and genetic differentiation of Haemaphysalis spp. is challenging. For instance, previous studies struggled to distinguish Haemaphysalis japonica and Haemaphysalis megaspinosa due to the dearth of nucleotide sequence polymorphisms in widely used barcoding genes. The classification of H. japonica japonica and its related sub-species Haemaphysalis japonica douglasi or Haemaphysalis jezoensis is also confused due to their high morphological similarity and a lack of molecular data that support the current classification. We used mitogenomes and microbiomes of H. japonica and H. megaspinosa to gain deeper insights into the phylogenetic relationships and genetic divergence between two species. Phylogenetic analyses of concatenated nucleotide sequences of protein-coding genes and ribosomal DNA genes distinguished H. japonica and H. megaspinosa as monophyletic clades, with further subdivision within the H. japonica clade. The 16S rRNA and NAD5 genes were valuable markers for distinguishing H. japonica and H. megaspinosa. Population genetic structure analyses indicated that genetic variation within populations accounted for a large proportion of the total variation compared to variation between populations. Microbiome analyses revealed differences in alpha and beta diversity between H. japonica and H. megaspinosa: H. japonica had the higher diversity. Coxiella sp., a likely endosymbiont, was found in both Haemaphysalis species. The abundance profiles of likely endosymbionts, pathogens, and commensals differed between H. japonica and H. megaspinosa: H. megaspinosa was more diverse.
(© 2024. The Author(s).)
Databáze: MEDLINE
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