Macrophage depletion overcomes human hematopoietic cell engraftment failure in zebrafish embryo.

Autor: El Omar R; University of Strasbourg, INSERM, EFS Grand-Est, BPPS UMR-S1255, Strasbourg, France.; Université de Lorraine, CITHEFOR, F-54505, Vandoeuvre Les Nancy, France., Abdellaoui N; University of Montpellier, VBIC, INSERM U1047, Montpellier, France., Coulibaly ST; University of Strasbourg, CNRS, Architecture et Réactivité de l'ARN, UPR 9002, Strasbourg, France.; ITI Innovec, Strasbourg, France., Fontenille L; AZELEAD SAS, Montpellier, France., Lanza F; University of Strasbourg, INSERM, EFS Grand-Est, BPPS UMR-S1255, Strasbourg, France., Gachet C; University of Strasbourg, INSERM, EFS Grand-Est, BPPS UMR-S1255, Strasbourg, France., Freund JN; ITI Innovec, Strasbourg, France.; University of Strasbourg, INSERM, IRFAC/UMR-S1113, Strasbourg, France.; INSERM, U1256 - NGERE, Université de Lorraine, 54500, Vandoeuvre-lès-Nancy, France., Negroni M; University of Strasbourg, CNRS, Architecture et Réactivité de l'ARN, UPR 9002, Strasbourg, France.; ITI Innovec, Strasbourg, France., Kissa K; University of Montpellier, VBIC, INSERM U1047, Montpellier, France.; AZELEAD SAS, Montpellier, France., Tavian M; University of Strasbourg, INSERM, EFS Grand-Est, BPPS UMR-S1255, Strasbourg, France. manuela.tavian@inserm.fr.; ITI Innovec, Strasbourg, France. manuela.tavian@inserm.fr.; University of Strasbourg, INSERM, IRFAC/UMR-S1113, Strasbourg, France. manuela.tavian@inserm.fr.
Jazyk: angličtina
Zdroj: Cell death & disease [Cell Death Dis] 2024 May 01; Vol. 15 (5), pp. 305. Date of Electronic Publication: 2024 May 01.
DOI: 10.1038/s41419-024-06682-x
Abstrakt: Zebrafish is widely adopted as a grafting model for studying human development and diseases. Current zebrafish xenotransplantations are performed using embryo recipients, as the adaptive immune system, responsible for host versus graft rejection, only reaches maturity at juvenile stage. However, transplanted primary human hematopoietic stem/progenitor cells (HSC) rapidly disappear even in zebrafish embryos, suggesting that another barrier to transplantation exists before the onset of adaptive immunity. Here, using a labelled macrophage zebrafish line, we demonstrated that engraftment of human HSC induces a massive recruitment of macrophages which rapidly phagocyte transplanted cells. Macrophages depletion, by chemical or pharmacological treatments, significantly improved the uptake and survival of transplanted cells, demonstrating the crucial implication of these innate immune cells for the successful engraftment of human cells in zebrafish. Beyond identifying the reasons for human hematopoietic cell engraftment failure, this work images the fate of human cells in real time over several days in macrophage-depleted zebrafish embryos.
(© 2024. The Author(s).)
Databáze: MEDLINE
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